10.1371/journal.pone.0016798.g006 Magali Plaisant Magali Plaisant Sophie Giorgetti-Peraldi Sophie Giorgetti-Peraldi Marike Gabrielson Marike Gabrielson Agnes Loubat Agnes Loubat Christian Dani Christian Dani Pascal Peraldi Pascal Peraldi Inhibition of Hh signaling inhibits colony formation of hMADS cells. Public Library of Science 2011 hh signaling inhibits hmads 2011-02-03 00:28:01 Figure https://plos.figshare.com/articles/figure/_Inhibition_of_Hh_signaling_inhibits_colony_formation_of_hMADS_cells_/471681 <p>Cells were plated at clonal density and maintained in medium supplemented with 10% FCS and FGF-2 (2.5 ng/ml) (Ctrl). One day after cell plating, 5 µM cyclopamine was added to the culture medium. (<b>A</b>): Photographs of colony formation of hMADS cells 3 weeks after treatment. A magnification of one colony is presented for each condition. (<b>B</b>): Photographs of colony formation of hMADS cells transfected with non relevant (NR) siRNA, Gli2 siRNA or only with hyperfect as a control (indicated “none” in the figure). Graphs represent the percentage of colony-forming cells in three independent experiments.</p>