10.6084/m9.figshare.c.3705607_D4.v1
Michael Chambers
Michael
Chambers
Wiam Turki-Judeh
Wiam
Turki-Judeh
Min Kim
Min
Kim
Kenny Chen
Kenny
Chen
Sean Gallaher
Sean
Gallaher
Albert Courey
Albert
Courey
Additional file 3: Figure S1. of Mechanisms of Groucho-mediated repression revealed by genome-wide analysis of Groucho binding and activity
Springer Nature
2017
Groucho
Transcriptional repression
Drosophila embryogenesis
ChIP-seq
RNA-seq
Chromatin-associated RNA-seq, RNA polymerase II pausing
2017-02-28 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_Figure_S1_of_Mechanisms_of_Groucho-mediated_repression_revealed_by_genome-wide_analysis_of_Groucho_binding_and_activity/4705990
Antibody validation (A) Chromatin isolated and sheared exactly as for the ChIP-seq analysis was subjected to immunoprecipitation with the indicated amounts (in μl) of affinity purified antibody against the Gro GP domain used for the ChIP-seq analysis, and then probed in a western blot with both an anti-Gro monoclonal antibody (mAb) or the anti-GP antibody. The band indicated by the asterisk is a cross-reacting protein that is recognized in the western blot but that is not efficiently immunoprecipitated by the anti-GP antibody. Ab HC – antibody heavy chain. (B) Heat map showing overlap (Jacard similarity coefficient [96]) between the peaks called in the duplicate ChIP-seq experiments at each time point. (C) Representative genome browser tracts comparing duplicate ChIP-seq experiments. (D and E) Comparison of Gro binding patterns obtained by ChIP-seq using our anti-GP antibody with that obtained by ChIP-chip (0–12 hr embryos; modENCODE #597) and ChIP-seq (white pre-pupae; modENCODE #4981) using independently derived antibodies [40]. (PDF 588 kb)