10.1371/journal.pone.0172571.g005
Li-Fu Li
Li-Fu
Li
Chung-Shu Lee
Chung-Shu
Lee
Chang-Wei Lin
Chang-Wei
Lin
Ning-Hung Chen
Ning-Hung
Chen
Li-Pang Chuang
Li-Pang
Chuang
Chen-Yiu Hung
Chen-Yiu
Hung
Yung-Yang Liu
Yung-Yang
Liu
Trichostatin A attenuated lung stretch-induced fibrogenic biomarkers.
Public Library of Science
2017
HDAC 4
staining
lung injury
ventilator-induced lung injury
plasminogen activator inhibitor
high-V T MV
HDAC 4 expression
MV-augmented lung inflammation
Akt-deficient C 57BL mice
TSA treatment attenuates high-V T MV-augmented EMT
ALI
MMP
Akt activation
MV-augmented bleomycin-induced EMT
attenuates ventilation-augmented epithelial-mesenchymal transition
Akt pathway Background Mechanical ventilation
1 gene expression
ARDS
matrix metalloproteinase -9
Akt pathways
2017-02-24 19:17:43
Figure
https://plos.figshare.com/articles/figure/Trichostatin_A_attenuated_lung_stretch-induced_fibrogenic_biomarkers_/4693444
<p>Five days after bleomycin treatment, representative photomicrographs (x400) with E-cadherin (E-cad, red or bright green), α-smooth muscle actin (α-SMA, red), and Hoechst (blue) immunofluorescent staining of paraffin lung sections (A) (n = 5 per group), and micro-computed tomography imaging (B) (n = 3 per group) were from nonventilated control mice and mice ventilated at a tidal volume of 6 mL/kg or 30 mL/kg for 5 h with room air. TSA 2 mg/kg was given intraperitoneally 1 h before mechanical ventilation. Positive red and bright green staining in the lung epithelium and interstitium is identified by arrows (n = 5 per group). The fibrotic scoring (C) was quantified as the average number of 10 nonoverlapping fields in Masson’s trichrome staining of paraffin lung sections (n = 5 per group). Scale bars represent 20 μm. ANOVA was used to assess the statistical significance of the differences, followed by multiple comparisons with a Scheffeˊs test, and a P value < 0.05 was considered statistically significant. * P< 0.05 versus the nonventilated control mice with bleomycin pretreatment; <b>†</b> P< 0.05 versus all other groups.</p>