10.1371/journal.pone.0172673.g005 Liubov Poshyvailo Liubov Poshyvailo Eric von Lieres Eric von Lieres Svyatoslav Kondrat Svyatoslav Kondrat Effect of channeling on reaction cascades with competing reactions. Public Library of Science 2017 side reactions substrate concentrations enzyme-catalyzed cascade reactions metabolite pool size non-channeled cascade reaction reaction rate 2017-02-24 19:19:22 Figure https://plos.figshare.com/articles/figure/Effect_of_channeling_on_reaction_cascades_with_competing_reactions_/4693420 <p>(a) Product formation velocity of the nonchanneled system, <i>v</i><sub><i>non</i></sub>, decreases as the degradation or consumption rate of intermediates, <i>k</i><sub>deg</sub>, increases. (b) Reaction velocity of the nonchanneled system falls off for increasing ‘degree of reversibility’ of the first reaction in the cascade, . In this case the reaction is in lieu of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0172673#pone.0172673.e001" target="_blank">Eq (1a)</a>. In both plots <i>v</i><sub><i>non</i></sub> is compared to the velocity of the channeled system, <i>v</i><sub><i>ch</i></sub>. The concentrations of enzymes and enzyme-enzyme complexes are equal in both systems on both panels, <i>i.e.</i> [E<sub><i>i</i></sub>] = [E<sub>1</sub>] = [E<sub>2</sub>] = [E<sub>12</sub>]; in panel (b) the ratio <i>v</i><sub><i>non</i></sub>/<i>v</i><sub><i>ch</i></sub> is independent of [E<sub><i>i</i></sub>]. Substrate concentration [S] = 90 nM, the degree of diffusion control is <i>γ</i><sub>1</sub> = <i>γ</i><sub><i>ch</i></sub> = 1 (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0172673#pone.0172673.e021" target="_blank">Eq (10)</a>), and the rate constants are the same as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0172673#pone.0172673.g001" target="_blank">Fig 1</a>. Thin dotted line denotes <i>v</i><sub><i>ch</i></sub> = <i>v</i><sub><i>non</i></sub>.</p>