%0 Figure %A Danielsson, Angelika %A Dzojic, Helena %A Rashkova, Victoria %A Cheng, Wing-Shing %A Essand, Magnus %D 2011 %T Differential gene expression in virus-transduced prostate cancer cells after 48 h of treatment with HDAC inhibitors. %U https://plos.figshare.com/articles/figure/_Differential_gene_expression_in_virus_transduced_prostate_cancer_cells_after_48_h_of_treatment_with_HDAC_inhibitors_/468633 %R 10.1371/journal.pone.0014700.g007 %2 https://ndownloader.figshare.com/files/798268 %K virus-transduced %K prostate %K cancer %K cells %K 48 %K hdac %X

LNCaP and PC-346C were transduced with Ad[i/PPT-LUC] and then incubated with 3 ng/ml FK228 or 5 mM VPA for 48 h. RNA was extracted, cDNA was produced and gene expression was evaluated by quantitative PCR (triplicate samples). Gene expression in FK228 and VPA-treated cells was related to untreated cells. A two-fold difference in gene expression was set as a limit for true difference. The expression of prostate-associated genes (AR, ARA24, PSA, PSMA, SRD5A1, SRD5A2 and TARP) was not considerably affected by the HDACi. The expression of neuroendocrine-associated genes (β-tubIII, CgA, NSE and SYP) was increased in HDACi-treated cells.

%I PLOS ONE