10.1371/journal.pone.0171274.g005
Shigeru Shimamura
Shigeru
Shimamura
Takashi Kaneko
Takashi
Kaneko
Genki Ozawa
Genki
Ozawa
Mamiko Nishino Matsumoto
Mamiko Nishino
Matsumoto
Takeru Koshiishi
Takeru
Koshiishi
Yoshihiro Takaki
Yoshihiro
Takaki
Chiaki Kato
Chiaki
Kato
Ken Takai
Ken
Takai
Takao Yoshida
Takao
Yoshida
Katsunori Fujikura
Katsunori
Fujikura
James P. Barry
James P.
Barry
Tadashi Maruyama
Tadashi
Maruyama
Electrophorograms of the RT-PCR products of NER genes in <i>Ca</i>. Vesicomyosocius okutanii (Vok) and the symbiont of <i>C</i>. <i>pacifica</i> (Cpac_S).
Public Library of Science
2017
RT-PCR
reductive genome evolution
GC
NER genes
RGE
DNA repair genes
7 clade II symbionts
Nucleotide Excision Repair
clade II
clade II symbionts
transcription-polymerase chain reaction
vesicomyid clam symbionts
uvrD p
vesicomyid clams lack
nucleotide excision repair
vesicomyid clams Intracellular thioautotrophic symbionts
2017-02-15 19:49:54
Figure
https://plos.figshare.com/articles/figure/Electrophorograms_of_the_RT-PCR_products_of_NER_genes_in_i_Ca_i_Vesicomyosocius_okutanii_Vok_and_the_symbiont_of_i_C_i_i_pacifica_i_Cpac_S_/4655374
<p>A, Cpac_S; B, Vok. Expression of NER-related genes was analyzed by RT-PCR. Lane 1, <i>uvrA</i>; lane 2, <i>uvrB</i> or corresponding DNA region; lane 3, <i>uvrC</i> or corresponding DNA region; lane 4, <i>uvrD</i>; lane 5, <i>uvrDp</i>; lane 6, <i>recA</i>; lane 7, <i>mfd</i>; lane 8, 16S rRNA gene; lane 9, 16S rRNA gene negative control (with RNase treatment before RT PCR). M, molecular markers. Primers and the predicted lengths of amplicons are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171274#pone.0171274.s003" target="_blank">S3 Table</a>. Red arrowheads indicate the position of a band where no signal was detected.</p>