10.1371/journal.pone.0171274.g005 Shigeru Shimamura Shigeru Shimamura Takashi Kaneko Takashi Kaneko Genki Ozawa Genki Ozawa Mamiko Nishino Matsumoto Mamiko Nishino Matsumoto Takeru Koshiishi Takeru Koshiishi Yoshihiro Takaki Yoshihiro Takaki Chiaki Kato Chiaki Kato Ken Takai Ken Takai Takao Yoshida Takao Yoshida Katsunori Fujikura Katsunori Fujikura James P. Barry James P. Barry Tadashi Maruyama Tadashi Maruyama Electrophorograms of the RT-PCR products of NER genes in <i>Ca</i>. Vesicomyosocius okutanii (Vok) and the symbiont of <i>C</i>. <i>pacifica</i> (Cpac_S). Public Library of Science 2017 RT-PCR reductive genome evolution GC NER genes RGE DNA repair genes 7 clade II symbionts Nucleotide Excision Repair clade II clade II symbionts transcription-polymerase chain reaction vesicomyid clam symbionts uvrD p vesicomyid clams lack nucleotide excision repair vesicomyid clams Intracellular thioautotrophic symbionts 2017-02-15 19:49:54 Figure https://plos.figshare.com/articles/figure/Electrophorograms_of_the_RT-PCR_products_of_NER_genes_in_i_Ca_i_Vesicomyosocius_okutanii_Vok_and_the_symbiont_of_i_C_i_i_pacifica_i_Cpac_S_/4655374 <p>A, Cpac_S; B, Vok. Expression of NER-related genes was analyzed by RT-PCR. Lane 1, <i>uvrA</i>; lane 2, <i>uvrB</i> or corresponding DNA region; lane 3, <i>uvrC</i> or corresponding DNA region; lane 4, <i>uvrD</i>; lane 5, <i>uvrDp</i>; lane 6, <i>recA</i>; lane 7, <i>mfd</i>; lane 8, 16S rRNA gene; lane 9, 16S rRNA gene negative control (with RNase treatment before RT PCR). M, molecular markers. Primers and the predicted lengths of amplicons are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171274#pone.0171274.s003" target="_blank">S3 Table</a>. Red arrowheads indicate the position of a band where no signal was detected.</p>