Unusual
Topological RNA Architecture with an Eight-Stranded
Helical Fragment Containing A‑, G‑, and U‑Tetrads
Chao-Da Xiao
Takumi Ishizuka
Xiao-Qing Zhu
Yue Li
Hiroshi Sugiyama
Yan Xu
10.1021/jacs.6b12274.s001
https://acs.figshare.com/articles/journal_contribution/Unusual_Topological_RNA_Architecture_with_an_Eight-Stranded_Helical_Fragment_Containing_A_G_and_U_Tetrads/4643782
Human
telomeric RNA performs various cellular functions such as
telomere length regulation, heterochromatin formation, and chromosome
end protection. Using a combination of nuclear magnetic resonance,
circular dichroism, and gel electrophoresis, we observed an unusual
topological structure formed by human telomere RNA r(GUUAGGGU). Our
results showed that every set of four strands formed a parallel G-quadruplex
as symmetry-related units containing four G-tetrads, two U-tetrads,
and one A-tetrad. An eight-stranded helical fragment containing A-,
G-, and U-tetrads provided a central intercalated scaffold that connected
two G-quadruplex units in an alternating antiparallel arrangement,
giving rise to a novel RNA architecture. This higher order RNA structure
is so stable that it would be surprising if similar structures do
not occur in nature. Our findings provide a new insight into the behavior
of human telomeric RNA molecules.
2017-02-08 00:00:00
chromosome end protection
telomere length regulation
topological structure
gel electrophoresis
G-quadruplex units
eight-stranded helical fragment
heterochromatin formation
symmetry-related units
Eight-Stranded Helical Fragment Containing
RNA molecules
order RNA structure
antiparallel arrangement
novel RNA architecture
intercalated scaffold
Unusual Topological RNA Architecture