10.1371/journal.pgen.1006561.g009
Katrin Schenk
Katrin
Schenk
Ana B. Hervás
Ana B.
Hervás
Thomas C. Rösch
Thomas C.
Rösch
Marc Eisemann
Marc
Eisemann
Bernhard A. Schmitt
Bernhard A.
Schmitt
Stephan Dahlke
Stephan
Dahlke
Luise Kleine-Borgmann
Luise
Kleine-Borgmann
Seán M. Murray
Seán
M. Murray
Peter L. Graumann
Peter
L. Graumann
Oscillation of DnaA-YFP<sup>sw</sup> in <i>E</i>. <i>coli</i>.
Public Library of Science
2017
half time binding
initiation control
replication origin regions
residence time
affinity binding sites
chromosomal binding sites
ORC
DNA replication DnaA
FRAP
2017-02-06 19:05:05
Figure
https://plos.figshare.com/articles/figure/Oscillation_of_DnaA-YFP_sup_sw_sup_in_i_E_i_i_coli_i_/4624465
<p>Fluorescence microscopy images of <i>E</i>. <i>coli</i> cells, A) expressing DnaA-YFP<sup>sw</sup> during exponential growth, B) 60 min after the addition of 200 μg/ml rifampicin, C) <i>B</i>. <i>subtilis</i> cells expressing TetR-YFP binding to a <i>tetO</i> array near <i>oriC</i>. Fluorescence intensities of two areas, each containing one fluorescence signal, were measured and normalized to the total fluorescence intensity of the cell. Intensities of both areas were plotted over time. Area 1 (black) and area 2 (white). Image sequences displaying the oscillation of DnaA-YFP<sup>sw</sup> (A–B) and the static TetR-YFP (C) are shown. White lines, cell borders; scale bars, 2 μm; white dashed lines, cell outlines; black open circle, area 1; green open circle; area 2. To visualize the correlation between the two measured areas they were plotted against each other (D–F). Correlation coefficients (r) were determined.</p>