TY - DATA T1 - Response of digestive organs of Hypsiboas albopunctatus (Anura: Hylidae) to benzo[α]pyrene PY - 2017/02/18 AU - Lara Zácari Fanali AU - Bruno Serra de Lacerda Valverde AU - Lilian Franco-Belussi AU - Diogo Provete AU - Classius de Oliveira UR - https://figshare.com/articles/journal_contribution/Response_of_digestive_organs_of_Hypsiboas_albopunctatus_Anura_Hylidae_to_benzo_pyrene/4620736 DO - 10.6084/m9.figshare.4620736.v4 L4 - https://ndownloader.figshare.com/files/7516357 L4 - https://ndownloader.figshare.com/files/7516360 L4 - https://ndownloader.figshare.com/files/7516369 L4 - https://ndownloader.figshare.com/files/7521691 L4 - https://ndownloader.figshare.com/files/7615987 KW - digestive system KW - intestinal epithelium KW - melanomacrophage, melanin KW - benzo[α]pyrene KW - anurans KW - Amphibians KW - Anatomy N2 - Here, we tested the dose dependent and short- and long-term effects of benzo[alpha]pyrene (BAP) on the thickness of small intestine and liver melanomacrophages (MMC) of Hypsiboas albopunctatus. We conducted an experiment with a fully-crossed 3x2 factorial design to answer these two questions. Male specimens were separated into groups injected with a subcutaneous solution containing either 3 or 7 mg/Kg of BAP. Specimens were killed either 72h or 168h after the first injection with BAP. Then, we measured the  the thickness of small intestine and the area occupied by MMCs in the liver. Experimental groups had 6 animals each. Our sampling units were pictures of histological sections (25 per animal). For the intestinal epithelium we only analyzed 5 out of the 6 animals. ER -