TY - DATA T1 - Overexpressing Nox1 and Nox4 increases ROS production. PY - 2017/02/02 AU - Benjamin J. Dickson AU - Mohamed I. Gatie AU - Danielle M. Spice AU - Gregory M. Kelly UR - https://plos.figshare.com/articles/figure/Overexpressing_i_Nox1_i_and_i_Nox4_i_increases_ROS_production_/4612015 DO - 10.1371/journal.pone.0170812.g006 L4 - https://ndownloader.figshare.com/files/7509364 KW - knockdown Nox 1 KW - NOX 4 levels KW - PrE KW - mouse F 9 cells KW - H 2 O 2 treatment KW - ROS KW - GATA KW - F 9 cells KW - reactive oxygen species KW - Nox 4 show KW - NADPH KW - antioxidants attenuates differentiation KW - RA KW - NOX 1-specific inhibitors KW - F 9 cells overexpressing Gata 6 KW - extraembryonic endoderm Mouse F 9 cells KW - NOX 1 KW - NOX 4 protein levels increase N2 - Total RNA was collected from F9 cells transfected with an empty vector (EV) control, pcDNA3.1-Nox1 or pcDNA3.1-Nox4 and cultured 4 days. (A) Nox1 and (B) Nox4 expression following transfection of pcDNA3.1-Nox1 or pcDNA3.1-Nox4, respectively, and relative to EV transfected cells. (C) NOX1 and (D) NOX4 protein levels following transfection of pcDNA3.1-Nox1 or pcDNA3.1-Nox4, respectively, relative to EV transfected cells. (E) ROS production using Amplex Red in F9 cells transfected with EV and treated with DMSO or RA, or transfected with pcDNA3.1-Nox1 or pcDNA3.1-Nox4. A total of 3 independent experiments were analyzed and results are presented as mean ± SEM. * denotes significance (P < 0.05) tested by a Student’s t-Test, whereas letters denote groups of significance (P < 0.05) tested by a One-Way ANOVA followed by a Tukey’s test. ER -