%0 Figure %A Dickson, Benjamin J. %A Gatie, Mohamed I. %A Spice, Danielle M. %A M. Kelly, Gregory %D 2017 %T Overexpressing Nox1 and Nox4 increases ROS production. %U https://plos.figshare.com/articles/figure/Overexpressing_i_Nox1_i_and_i_Nox4_i_increases_ROS_production_/4612015 %R 10.1371/journal.pone.0170812.g006 %2 https://ndownloader.figshare.com/files/7509364 %K knockdown Nox 1 %K NOX 4 levels %K PrE %K mouse F 9 cells %K H 2 O 2 treatment %K ROS %K GATA %K F 9 cells %K reactive oxygen species %K Nox 4 show %K NADPH %K antioxidants attenuates differentiation %K RA %K NOX 1-specific inhibitors %K F 9 cells overexpressing Gata 6 %K extraembryonic endoderm Mouse F 9 cells %K NOX 1 %K NOX 4 protein levels increase %X

Total RNA was collected from F9 cells transfected with an empty vector (EV) control, pcDNA3.1-Nox1 or pcDNA3.1-Nox4 and cultured 4 days. (A) Nox1 and (B) Nox4 expression following transfection of pcDNA3.1-Nox1 or pcDNA3.1-Nox4, respectively, and relative to EV transfected cells. (C) NOX1 and (D) NOX4 protein levels following transfection of pcDNA3.1-Nox1 or pcDNA3.1-Nox4, respectively, relative to EV transfected cells. (E) ROS production using Amplex Red in F9 cells transfected with EV and treated with DMSO or RA, or transfected with pcDNA3.1-Nox1 or pcDNA3.1-Nox4. A total of 3 independent experiments were analyzed and results are presented as mean ± SEM. * denotes significance (P < 0.05) tested by a Student’s t-Test, whereas letters denote groups of significance (P < 0.05) tested by a One-Way ANOVA followed by a Tukey’s test.

%I PLOS ONE