TY - DATA T1 - hUCBSC treatment downregulates cyclin D1 in vitro and in vivo. PY - 2011/03/24 AU - Kiran Kumar Velpula AU - Venkata Ramesh Dasari AU - Andrew J. Tsung AU - Christopher S. Gondi AU - Jeffrey D. Klopfenstein AU - Sanjeeva Mohanam AU - Jasti S. Rao UR - https://plos.figshare.com/articles/figure/_hUCBSC_treatment_downregulates_cyclin_D1_in_vitro____and_in_vivo_/460948 DO - 10.1371/journal.pone.0018017.g005 L4 - https://ndownloader.figshare.com/files/790584 KW - downregulates KW - cyclin KW - d1 N2 - (A) Immunocytochemistry of single and co-cultures of U251 and 5310 cells for the expression of cyclin D1 and CD81. Cyclin D1 is conjugated with Alexa Fluor-594 (red), and CD81 is conjugated with Alexa Flour-488 (green). Inset pictures show magnified images. Bar = 200 µm (B) Nude mice with pre-established intracranial human glioma tumors (U251 or 5310) were treated with hUCBSC by intracranial injection (2×105). Fourteen days after hUCBSC administration, the brains were harvested and sectioned and immunoprobed for cyclin D1 and appropriate secondary antibodies. Inset pictures show magnified images. Bar  = 200 µm. Each experiment was performed in triplicates with each sample (n = 3). ER -