10.1371/journal.pone.0018017.g005 Kiran Kumar Velpula Kiran Kumar Velpula Venkata Ramesh Dasari Venkata Ramesh Dasari Andrew J. Tsung Andrew J. Tsung Christopher S. Gondi Christopher S. Gondi Jeffrey D. Klopfenstein Jeffrey D. Klopfenstein Sanjeeva Mohanam Sanjeeva Mohanam Jasti S. Rao Jasti S. Rao hUCBSC treatment downregulates cyclin D1 <i>in vitro</i> and <i>in vivo</i>. Public Library of Science 2011 downregulates cyclin d1 2011-03-24 00:15:48 Figure https://plos.figshare.com/articles/figure/_hUCBSC_treatment_downregulates_cyclin_D1_in_vitro____and_in_vivo_/460948 <p>(<b>A</b>) Immunocytochemistry of single and co-cultures of U251 and 5310 cells for the expression of cyclin D1 and CD81. Cyclin D1 is conjugated with Alexa Fluor-594 (red), and CD81 is conjugated with Alexa Flour-488 (green). Inset pictures show magnified images. Bar = 200 µm (<b>B</b>) Nude mice with pre-established intracranial human glioma tumors (U251 or 5310) were treated with hUCBSC by intracranial injection (2×10<sup>5</sup>). Fourteen days after hUCBSC administration, the brains were harvested and sectioned and immunoprobed for cyclin D1 and appropriate secondary antibodies. Inset pictures show magnified images. Bar  = 200 µm. Each experiment was performed in triplicates with each sample (n = 3).</p>