TY - DATA T1 - Functional Characterization of Cotton GaMYB62L, a Novel R2R3 TF in Transgenic Arabidopsis PY - 2017/01/26 AU - Hamama Islam Butt AU - Zhaoen Yang AU - Eryong Chen AU - Ge Zhao AU - Qian Gong AU - Zuoren Yang AU - Xueyan Zhang AU - Fuguang Li UR - https://plos.figshare.com/articles/dataset/Functional_Characterization_of_Cotton_GaMYB62L_a_Novel_R2R3_TF_in_Transgenic_Arabidopsis/4608397 DO - 10.1371/journal.pone.0170578 L4 - https://ndownloader.figshare.com/files/7488832 L4 - https://ndownloader.figshare.com/files/7488838 L4 - https://ndownloader.figshare.com/files/7488847 L4 - https://ndownloader.figshare.com/files/7488868 L4 - https://ndownloader.figshare.com/files/7488883 KW - 29A KW - WT KW - RD KW - R 2R KW - Novel R 2R TF KW - seed germination rate KW - marker genes KW - Cotton GaMYB 62L KW - GaMYB 62L cDNA KW - GaMYB 62L protein KW - MW KW - water loss rate KW - COR KW - GaMYB 62L overexpression KW - R 2R MYB TFs KW - overexpressed GaMYB 62L lines KW - plant survival rates KW - GaMYB 62L KW - ADH KW - CDS KW - 292- residue polypeptide KW - Transgenic Arabidopsis Drought stress KW - root elongation assay KW - abiotic stress tolerance KW - SANT KW - ABA KW - 8.91. Overexpressed GaMYB 62L KW - 15A N2 - Drought stress can trigger the production of ABA in plants, in response to adverse conditions, which induces the transcript of stress-related marker genes. The R2R3 MYB TFs are implicated in regulation of various plants developmental, metabolic and multiple environmental stress responses. Here, a R2R3-MYB cloned gene, GaMYB62L, was transformed in Arabidopsis and was functionally characterized. The GaMYB62L protein contains two SANT domains with a conserved R2R3 imperfect repeats. The GaMYB62L cDNA is 1,017 bp with a CDS of 879, encodes a 292-residue polypeptide with MW of 38.78 kD and a pI value of 8.91. Overexpressed GaMYB62L transgenic Arabidopsis have increased proline and chlorophyll content, superior seed germination rate under salt and osmotic stress, less water loss rate with reduced stomatal apertures, high drought avoidance as compared to WT on water deprivation and also significant plant survival rates at low temperature. In addition, overexpressed GaMYB62L lines were more sensitive to ABA mediated germination and root elongation assay. Moreover, ABA induced GaMYB62L overexpression, enhanced the expression of ABA stress related marker genes like RD22, COR15A, ADH1, and RD29A. Together, overexpression of GaMYB62L suggested having developed better drought, salt and cold tolerance in transgenic Arabidopsis and thus presented it as a prospective candidate gene to achieve better abiotic stress tolerance in cotton crop. ER -