TY - DATA T1 - PACAP counteracts ketamine induced apoptosis and ER stress. PY - 2017/01/26 AU - Shiva Mansouri AU - Ingrid Agartz AU - Sven-Ove Ögren AU - Cesare Patrone AU - Mathias Lundberg UR - https://plos.figshare.com/articles/figure/PACAP_counteracts_ketamine_induced_apoptosis_and_ER_stress_/4605388 DO - 10.1371/journal.pone.0170496.g004 L4 - https://ndownloader.figshare.com/files/7484827 KW - RT-PCR KW - AMPA pathway activation KW - mTOR Pathway Activation Ketamine administration KW - ketamine impairs NSC viability KW - schizophrenia KW - Western blot analysis KW - NSC viability KW - ER KW - AMPA receptor activation KW - Adult neurogenesis dysregulation KW - PACAP Protects Adult Neural Stem Cells KW - adenylate cyclase-activating polypeptide N2 - NSCs were plated as single cells and treated with 400 μM ketamine alone or with 100 nM PACAP and 400 μM ketamine. After 24 hours incubation, cells were harvested for Western blot analysis (A, B) or harvested for mRNA extraction and quantitative RT-PCR experiments (C). To obtain quantitative measurements Bcl-2 protein levels and cleaved caspase-3 were normalized against coomassie blue. Data are shown as mean ±SEM (A, n = 5–6; B, n = 3–5, C, n = 4–6). Kruskal-Wallis followed by Dunn’s test. Differences were considered significant at P<0.05. * denotes P<0.05 compared with control, # denotes P<0.05 compared to 400 μM ketamine. ER -