10.6084/m9.figshare.4597828.v1
Tan Phat Nguyen
Tan
Phat Nguyen
Cong Luan Tran
Cong Luan
Tran
Chi Hung Vuong
Chi Hung
Vuong
Thi Hong Tuoi Do
Thi Hong
Tuoi
Tien Dung Le
Tien
Dung Le
Dinh Tri Mai
Dinh
Tri Mai
Nhat Minh Phan
Nhat
Minh Phan
Flavonoids with hepatoprotective activity from the leaves of <i>Cleome viscosa</i> L.
Taylor & Francis Group
2017
Cleome viscosa
capparaceae
visconoside C
cytotoxicity activity
hepatoprotective activity
2017-01-31 07:46:19
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Flavonoids_with_hepatoprotective_activity_from_the_leaves_of_i_Cleome_viscosa_i_L_/4597828
<p>One new flavonol glycoside named visconoside C (<b>1</b>), together with seven known flavonol glycosides, quercetin 3-O-β-d-glucopyranoside 7-O-α-l-rhamnopyranoside (<b>2</b>), quercetin 7-O-α-l-rhamnopyranoside (<b>3</b>), astragalin (<b>4</b>), kaempferol 3-<i>O</i>-(4-<i>O</i>-acetyl)-<i>α</i>-l-rhamnopyranoside (<b>5</b>), kaempferol 7-<i>O</i>-<i>α</i>-l-rhamnopyranoside (<b>6</b>), kaempferitrin (<b>7</b>) and kaempferol 3-<i>O</i>-<i>β</i>-d-glucopyranoside 7-<i>O</i>-<i>α</i>-l-rhamnopyranoside (<b>8</b>) were isolated by various chromatography methods from the leaves of <i>Cleome viscosa</i> L. Their structures were elucidated by IR, UV, HR-ESI-MS and NMR (1D & 2D) experiments. The cytotoxicity and hepatoprotective activities using HepG2 human hepatoma cell line of <b>1</b> were measured by MTT assay. At the concentration of 25 μM and 50 μM, <b>1</b> showed cytotoxic activity against HepG2 cells (cell viability was decreased to 22.2 and 23.0%, respectively, compared with doxorubicin control), while at the concentration of 100 μM, <b>1</b> showed hepatoprotective activity against CCl<sub>4</sub>-induced hepatotoxicity on HepG2 cells (34.3%, compared with quercetin control).</p>