10.6084/m9.figshare.4597828.v1 Tan Phat Nguyen Tan Phat Nguyen Cong Luan Tran Cong Luan Tran Chi Hung Vuong Chi Hung Vuong Thi Hong Tuoi Do Thi Hong Tuoi Tien Dung Le Tien Dung Le Dinh Tri Mai Dinh Tri Mai Nhat Minh Phan Nhat Minh Phan Flavonoids with hepatoprotective activity from the leaves of <i>Cleome viscosa</i> L. Taylor & Francis Group 2017 Cleome viscosa capparaceae visconoside C cytotoxicity activity hepatoprotective activity 2017-01-31 07:46:19 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Flavonoids_with_hepatoprotective_activity_from_the_leaves_of_i_Cleome_viscosa_i_L_/4597828 <p>One new flavonol glycoside named visconoside C (<b>1</b>), together with seven known flavonol glycosides, quercetin 3-O-β-d-glucopyranoside 7-O-α-l-rhamnopyranoside (<b>2</b>), quercetin 7-O-α-l-rhamnopyranoside (<b>3</b>), astragalin (<b>4</b>), kaempferol 3-<i>O</i>-(4-<i>O</i>-acetyl)-<i>α</i>-l-rhamnopyranoside (<b>5</b>), kaempferol 7-<i>O</i>-<i>α</i>-l-rhamnopyranoside (<b>6</b>), kaempferitrin (<b>7</b>) and kaempferol 3-<i>O</i>-<i>β</i>-d-glucopyranoside 7-<i>O</i>-<i>α</i>-l-rhamnopyranoside (<b>8</b>) were isolated by various chromatography methods from the leaves of <i>Cleome viscosa</i> L. Their structures were elucidated by IR, UV, HR-ESI-MS and NMR (1D & 2D) experiments. The cytotoxicity and hepatoprotective activities using HepG2 human hepatoma cell line of <b>1</b> were measured by MTT assay. At the concentration of 25 μM and 50 μM, <b>1</b> showed cytotoxic activity against HepG2 cells (cell viability was decreased to 22.2 and 23.0%, respectively, compared with doxorubicin control), while at the concentration of 100 μM, <b>1</b> showed hepatoprotective activity against CCl<sub>4</sub>-induced hepatotoxicity on HepG2 cells (34.3%, compared with quercetin control).</p>