TY - DATA T1 - Siglec-1 colocalizes with p24 in VCCs of HIV-1-infected MDMs. PY - 2017/01/27 AU - Jason E. Hammonds AU - Neal Beeman AU - Lingmei Ding AU - Sarah Takushi AU - Ashwanth C. Francis AU - Jaang-Jiun Wang AU - Gregory B. Melikyan AU - Paul Spearman UR - https://plos.figshare.com/articles/figure/Siglec-1_colocalizes_with_p24_in_VCCs_of_HIV-1-infected_MDMs_/4592770 DO - 10.1371/journal.ppat.1006181.g004 L4 - https://ndownloader.figshare.com/files/7490452 KW - Siglec -1-mediated virion KW - particle uptake KW - macrophage-to-T cell transmission KW - virus-containing compartment KW - VCC formation KW - HIV KW - cell surface lectin KW - non-infectious virus-like particles KW - intracellular compartment KW - target T lymphocytes KW - autologous T cells KW - ganglioside-containing virus-like particles N2 - (A) MDMs were infected with a biological stock of HIV-1 BaL at a TCID50 of 0.5/cell. 10 days post-infection cells were fixed and immunolabeled for HIV-1 p24 (green), CD9 (red), tetherin (magenta) and DAPI co-stained. (B) Same immunostaining profile as (A) with the exception of Siglec-1 (red) in place of CD9. Size bar = 10 μm. (C) MDMs were infected with VSV-G pseudotyped NL4.3 and NLUdel (D) at a TCID50 of 2.0/cell. 10 days post-infection cells were fixed and immunostained as described in (B). All size bars = 10 μm. Representative maximum intensity deconvolved images shown. ER -