Supplementary Figure S3. Apoptosis correlates with hypoxic cores in larger spheroids. from Measurement of oxygen tension within mesenchymal stem cell spheroids
Kaitlin C. Murphy
Ben P. Hung
Stephen Browne-Bourne
Dejie Zhou
Jessica Yeung
Damian C. Genetos
J. Kent Leach
10.6084/m9.figshare.4591642.v1
https://rs.figshare.com/articles/journal_contribution/Supplementary_Figure_S3_Apoptosis_correlates_with_hypoxic_cores_in_larger_spheroids_from_Measurement_of_oxygen_tension_within_mesenchymal_stem_cell_spheroids/4591642
(A) Annexin V visibly stained larger spheroids, and a statistically significant apoptotic core appears beginning with spheroids containing 250,000 cells. Annexin V, red; DAPI, blue. (B) Quantification of apoptosis index, calculated by the area of positive Annexin V staining in the core relative to the periphery (n=3). Groups with no significance are linked by the same letters, while groups with significance do not share the same letters.
2017-01-27 11:08:48
oxygen tension
diffusion
spheroid
hypoxia
mesenchymal stem cell