MOESM4 of TDP-43 protein variants as biomarkers in amyotrophic lateral sclerosis WilliamsStephanie KhanGalam HarrisBrent RavitsJohn SierksMichael 2017 Additional file 4: Figure S4. Analysis of Purified Soluble Antibody Fragments by Western Blot. Following Ni–NTA purification of 17 of the 23 scFvs, analysis by Western blotting indicated the presence of the expected ~28 kDa band corresponding to a full length scFv (except for ALS-TDP10). The estimated molecular weight for each scFv is indicated below their respective band.