TY - DATA T1 - Loss of Syk activity does not attenuate airway inflammation and remodeling or total lung collagen content in the chronic HDM-challenge model. PY - 2017/01/20 AU - Sepehr Salehi AU - Xiaomin Wang AU - Stephen Juvet AU - Jeremy A. Scott AU - Chung-Wai Chow UR - https://plos.figshare.com/articles/figure/Loss_of_Syk_activity_does_not_attenuate_airway_inflammation_and_remodeling_or_total_lung_collagen_content_in_the_chronic_HDM-challenge_model_/4577317 DO - 10.1371/journal.pone.0163614.g006 L4 - https://ndownloader.figshare.com/files/7471555 KW - 8- week mouse model KW - Chronic Mouse Model KW - HDM-specific IgE levels KW - Allergic Airways Inflammation Background Asthma KW - bronchoalveolar lavage cell counts KW - gene expression analysis KW - GSK 143-treated mice KW - CXCL KW - house dust mite KW - Results MCh-induced AHR KW - BALB KW - Syk Regulates Neutrophilic Airway Hyper-Responsiveness KW - Conclusion Syk inhibitors KW - airways inflammation KW - IL KW - Syk modulates methacholine-induced airways contractility N2 - Immunostaining for Syk (green) revealed expression in airway epithelia (arrow) and leukocytes (*) under basal Saline conditions and following HDM exposure in all groups of mice (A-E, G) except the Syk-deficient (Sykdel/del) mice (F, H). Following HDM exposure, increased peribronchial leukocyte recruitment (DAPI staining, blue) was also observed (C,D,G,H), as was an increase in α-smooth muscle actin (stained red); this pattern was observed in the control Veh and Sykflox/flox mice (C,G) as well as the GSK-treated and Syk-deficient Sykdel/del mice (D,H). Images are representative of 4 mice/group. Bar represents 100 μm. I: Quantitative analysis revealed a 2-fold increase in the total lung collagen content in the HDM mice when compared with Saline control in all groups of mice (*p<0.05, HDM vs. Saline, 2 way ANOVA, n = 4/group). No differences were observed between Sykflox/flox and Sykdel/del mice, nor between BALB/c mice treated with Veh or GSK143. ER -