TY - DATA T1 - Supplementary Material for: Mini-Laparoscopy Guided Liver Biopsy Increases Diagnostic Accuracy in Acute Liver Failure PY - 2017/01/20 AU - DechĂȘne A. AU - Sowa J.-P. AU - Schlattjan M. AU - Wree A. AU - Blomeyer S. AU - Best J. AU - Maldonado E.J. AU - Bechmann L.P. AU - Gerken G. AU - Baba H.A. AU - Syn W.-K. AU - Canbay A UR - https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Mini-Laparoscopy_Guided_Liver_Biopsy_Increases_Diagnostic_Accuracy_in_Acute_Liver_Failure/4569889 DO - 10.6084/m9.figshare.4569889.v1 L4 - https://ndownloader.figshare.com/files/7401922 L4 - https://ndownloader.figshare.com/files/7401925 L4 - https://ndownloader.figshare.com/files/7401928 KW - Mini laparoscopy KW - Acute liver failure KW - Liver cirrhosis KW - Liver biopsy KW - Coagulopathy KW - Cell death N2 - Background/Aims: For diagnosis, prognosis, and treatment of acute liver failure (ALF), macroscopic evaluation and histological assessment of the liver are important. Due to impaired coagulation in ALF, the risk of bleeding is high after a percutaneous liver biopsy. Our aims were to assess (i) safety and benefit of mini laparoscopy (ML) in patients with ALF and (ii) the potential utility of histological markers in ALF prognosis. Methods: ML was performed in 39 patients with ALF to assess liver surface and to obtain a liver biopsy. Serological markers of liver injury and immunohistochemical detection of cell death and proliferation were compared to a non-ALF group (n = 10). Results: Liver biopsies were successfully performed in all patients with no significant complications. All patients had markedly elevated M30 and M65 levels in the serum. In the liver, M30 and Ki67 immune-reactive cells were more abundant in those with ALF. Importantly, there were significantly more Ki67-positive cells but fewer M30-positive cells in livers of ALF patients who recovered spontaneously. Conclusion: ML with liver biopsy in patients with ALF and severe coagulopathy is safe. Immunohistochemical detection of liver cell death and regeneration may identify individuals who would recover spontaneously or who would need a liver transplant. ER -