10.6084/m9.figshare.c.3667594_D4.v1
Amirali Kia
Amirali
Kia
Christian Gloeckner
Christian
Gloeckner
Trina Osothprarop
Trina
Osothprarop
Niall Gormley
Niall
Gormley
Erin Bomati
Erin
Bomati
Michelle Stephenson
Michelle
Stephenson
Igor Goryshin
Igor
Goryshin
Molly He
Molly
He
Additional file 4: Figure S3. of Improved genome sequencing using an engineered transposase
Springer Nature
2017
Tn 5 tagmentation
figure
9 base pairs overlap
genome sequencing
symmetry
Fig . 3a
bias plots
. 3a
Tn 5-B form
DNA
bias plot
position P
Tn 5 transposase monomers
Tn 5-A
position 5.
position 10- P
PDF 113Â kb
positions 1
transposase Schematic representation
bottom strands
sequencing results
2017-01-17 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_4_Figure_S3_of_Improved_genome_sequencing_using_an_engineered_transposase/4560298
Schematic representation of Tn5 tagmentation. There is 9 base pairs overlap between top and bottom strands. Two Tn5 transposase monomers, Tn5-A and Tn5-B form a dimer and tagment a double stranded DNA. Forward and reverse strands are shown in the figure. A typical bias plot from sequencing of B. cereus is also shown in the figure (refer to Fig. 3a). For every base at position P (where P is between 1 and 9) in a read, theoretically there is another read in the sequencing results that has complementary nucleotide to that base at position 10-P. This results in a symmetry between positions 1 and 9 in the bias plots, and the center of the symmetry will be at position 5. (PDF 113 kb)