10.6084/m9.figshare.c.3667594_D4.v1 Amirali Kia Amirali Kia Christian Gloeckner Christian Gloeckner Trina Osothprarop Trina Osothprarop Niall Gormley Niall Gormley Erin Bomati Erin Bomati Michelle Stephenson Michelle Stephenson Igor Goryshin Igor Goryshin Molly He Molly He Additional file 4: Figure S3. of Improved genome sequencing using an engineered transposase Springer Nature 2017 Tn 5 tagmentation figure 9 base pairs overlap genome sequencing symmetry Fig . 3a bias plots . 3a Tn 5-B form DNA bias plot position P Tn 5 transposase monomers Tn 5-A position 5. position 10- P PDF 113  kb positions 1 transposase Schematic representation bottom strands sequencing results 2017-01-17 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_4_Figure_S3_of_Improved_genome_sequencing_using_an_engineered_transposase/4560298 Schematic representation of Tn5 tagmentation. There is 9 base pairs overlap between top and bottom strands. Two Tn5 transposase monomers, Tn5-A and Tn5-B form a dimer and tagment a double stranded DNA. Forward and reverse strands are shown in the figure. A typical bias plot from sequencing of B. cereus is also shown in the figure (refer to Fig. 3a). For every base at position P (where P is between 1 and 9) in a read, theoretically there is another read in the sequencing results that has complementary nucleotide to that base at position 10-P. This results in a symmetry between positions 1 and 9 in the bias plots, and the center of the symmetry will be at position 5. (PDF 113 kb)