%0 Figure %A Chopra-Dewasthaly, Rohini %A Korb, Melanie %A Brunthaler, René %A Ertl, Reinhard %D 2017 %T Validation of RNA-Seq results by RT-qPCR. %U https://plos.figshare.com/articles/figure/Validation_of_RNA-Seq_results_by_RT-qPCR_/4548304 %R 10.1371/journal.pone.0170015.g005 %2 https://ndownloader.figshare.com/files/7458811 %K Illumina RNA-sequencing %K Sheep Mammary Gland Transcriptome %K mycoplasma mastitis %K host response %K gene Ontology %K subclinical state %K Similar RNA-Seq analyses %K DEG %K Several differentially %K Spleen cells %K subacute mastitis %K mycoplasma defense responses %K agalactiae infection %K expression profiles %K control animals %K host transcriptomics %K study reports transcriptome %K intervention strategies %K host-pathogen interplay %K RT-qPCR analyses %K udders %K transcriptome responses %K mechanism %K Comprehensive RNA-Seq Profiling %K agalactiae type strain PG 2 %K Experimental Mycoplasma agalactiae Infection Mycoplasma agalactiae %K immune-inflammatory responses %K spleen cells %X

Fold changes (M. agalactiae vs. non-infected) obtained by RT-qPCR were compared with the sequencing results for seven DEGs. Bars represent the mean values ± SEM of 6 replicates per group. Stars indicate significant expression changes.

%I PLOS ONE