Validation of RNA-Seq results by RT-qPCR.
Rohini Chopra-Dewasthaly
Melanie Korb
René Brunthaler
Reinhard Ertl
10.1371/journal.pone.0170015.g005
https://plos.figshare.com/articles/figure/Validation_of_RNA-Seq_results_by_RT-qPCR_/4548304
<p>Fold changes (<i>M</i>. <i>agalactiae</i> vs. non-infected) obtained by RT-qPCR were compared with the sequencing results for seven DEGs. Bars represent the mean values ± SEM of 6 replicates per group. Stars indicate significant expression changes.</p>
2017-01-12 17:32:48
Illumina RNA-sequencing
Sheep Mammary Gland Transcriptome
mycoplasma mastitis
host response
gene Ontology
subclinical state
Similar RNA-Seq analyses
DEG
Several differentially
Spleen cells
subacute mastitis
mycoplasma defense responses
agalactiae infection
expression profiles
control animals
host transcriptomics
study reports transcriptome
intervention strategies
host-pathogen interplay
RT-qPCR analyses
udders
transcriptome responses
mechanism
Comprehensive RNA-Seq Profiling
agalactiae type strain PG 2
Experimental Mycoplasma agalactiae Infection Mycoplasma agalactiae
immune-inflammatory responses
spleen cells