Validation of RNA-Seq results by RT-qPCR. Rohini Chopra-Dewasthaly Melanie Korb René Brunthaler Reinhard Ertl 10.1371/journal.pone.0170015.g005 https://plos.figshare.com/articles/figure/Validation_of_RNA-Seq_results_by_RT-qPCR_/4548304 <p>Fold changes (<i>M</i>. <i>agalactiae</i> vs. non-infected) obtained by RT-qPCR were compared with the sequencing results for seven DEGs. Bars represent the mean values ± SEM of 6 replicates per group. Stars indicate significant expression changes.</p> 2017-01-12 17:32:48 Illumina RNA-sequencing Sheep Mammary Gland Transcriptome mycoplasma mastitis host response gene Ontology subclinical state Similar RNA-Seq analyses DEG Several differentially Spleen cells subacute mastitis mycoplasma defense responses agalactiae infection expression profiles control animals host transcriptomics study reports transcriptome intervention strategies host-pathogen interplay RT-qPCR analyses udders transcriptome responses mechanism Comprehensive RNA-Seq Profiling agalactiae type strain PG 2 Experimental Mycoplasma agalactiae Infection Mycoplasma agalactiae immune-inflammatory responses spleen cells