10.6084/m9.figshare.4542745.v1
Kumaran S.
Kumaran
S.
Samantha K.S.
Samantha
K.S.
Halagowder D.
Halagowder
D.
Supplementary Material for: Does ß-Catenin Cross-Regulate NFκB Signalling in Pancreatic Cancer and Chronic Pancreatitis?
Karger Publishers
2017
β-Catenin
NFκB
Chronic pancreatitis
Pancreatic cancer
2017-01-12 13:02:13
Dataset
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Does_-Catenin_Cross-Regulate_NF_B_Signalling_in_Pancreatic_Cancer_and_Chronic_Pancreatitis_/4542745
<p><b><i>Background:</i></b> It is not clear by which mechanism nuclear
factor-kappaB (NFκB) induces cell proliferation and escapes from the
apoptotic pathway in pancreatic carcinogenesis. This study aimed to
investigate ß<i>-</i>catenin and NFκB signalling in chronic pancreatitis and pancreatic cancer. <b><i>Materials and Methods:</i></b>
On tissue samples of chronic pancreatitis and pancreatic cancer, we
performed immunohistochemistry for detecting the expression of tumour
necrosis factor alpha (TNFa), ß<i>-</i>catenin and NFκB, Western blot
for TNFa, NFκB, ß-catenin, c-Myc and FasL, co-immunoprecipitation for
ß-catenin with NFκB and RT-PCR for cyclin D1, c-Myc and Fas. <b><i>Results:</i></b>
TNFa and NFκB expression was increased in chronic pancreatitis and
pancreatic cancer. ß-Catenin and cyclin D1 expression was intense in
pancreatic cancer and moderate in chronic pancreatitis. Of the NFκB
response elements, the expression of pro-proliferative c-Myc is intense
in pancreatic cancer and moderate in chronic pancreatitis, but the
pro-apoptotic factors, Fas and FasL were down-regulated in pancreatic
cancer and moderately expressed in chronic pancreatitis. The
co-immunoprecipitation results showed a significant interaction of
ß-catenin with NFκB in pancreatic cancer and a non-significant
interaction in chronic pancreatitis. <b><i>Conclusions:</i></b> ß<i>-</i>Catenin
might cross-regulate NFκB by interrupting the balance of cell death and
cell survival via recruiting NFκB into the cell survival pathway alone
in most cases of pancreatic cancer and some cases of chronic
pancreatitis.</p>