10.6084/m9.figshare.4542694.v1 Hirpara D.G. Hirpara D.G. Gajera H.P. Gajera H.P. Hirpara H.Z. Hirpara H.Z. Golakiya B.A. Golakiya B.A. Supplementary Material for: Antipathy of Trichoderma against Sclerotium rolfsii Sacc.: Evaluation of Cell Wall-Degrading Enzymatic Activities and Molecular Diversity Analysis of Antagonists Karger Publishers 2017 Sclerotium rolfsii Trichoderma Biocontrol Mycoparasitism Simple sequence repeat polymorphism 2017-01-12 12:34:26 Dataset https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Antipathy_of_Trichoderma_against_Sclerotium_rolfsii_Sacc_Evaluation_of_Cell_Wall-Degrading_Enzymatic_Activities_and_Molecular_Diversity_Analysis_of_Antagonists/4542694 <p>The fungus <i>Trichoderma</i> is a teleomorph of the <i>Hypocrea</i> genus and associated with biological control of plant diseases. The microscopic, biochemical, and molecular characterization of <i>Trichoderma </i>was carried out and evaluated for in vitro antagonistic activity against the fungal pathogen <i>Sclerotium rolfsii</i> causing stem rot disease in groundnut. In total, 11 isolates of <i>Trichoderma</i> were examined for antagonism at 6 and 12 days after inoculation (DAI). Out of 11, <i>T. virens </i>NBAII Tvs12 evidenced the highest (87.91%) growth inhibition of the test pathogen followed by <i>T. koningii</i> MTCC 796 (67.03%), <i>T. viride </i>NBAII Tv23 (63.74%), and <i>T. harzianum </i>NBAII Th1 (60.44%). Strong mycoparasitism was observed in the best antagonist Tvs12 strain during 6-12 DAI. The specific activity of cell wall-degrading enzymes - chitinase and β-1,3-glucanase - was positively correlated with growth inhibition of the test pathogen. In total, 18 simple sequence repeat (SSR) polymorphisms were reported to amplify 202 alleles across 11 <i>Trichoderma </i>isolates. The average polymorphism information content for SSR markers was found to be 0.80. The best antagonist Tvs 12 was identified with 7 unique SSR alleles amplified by 5 SSR markers. Clustering patterns of 11 <i>Trichoderma </i>strains showed the best antagonist <i>T. virens</i> NBAII Tvs 12 outgrouped with a minimum 3% similarity from the rest of <i>Trichoderma.</i></p>