10.6084/m9.figshare.4542694.v1
Hirpara D.G.
Hirpara
D.G.
Gajera H.P.
Gajera
H.P.
Hirpara H.Z.
Hirpara
H.Z.
Golakiya B.A.
Golakiya
B.A.
Supplementary Material for: Antipathy of Trichoderma against Sclerotium rolfsii Sacc.: Evaluation of Cell Wall-Degrading Enzymatic Activities and Molecular Diversity Analysis of Antagonists
Karger Publishers
2017
Sclerotium rolfsii
Trichoderma
Biocontrol
Mycoparasitism
Simple sequence repeat polymorphism
2017-01-12 12:34:26
Dataset
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Antipathy_of_Trichoderma_against_Sclerotium_rolfsii_Sacc_Evaluation_of_Cell_Wall-Degrading_Enzymatic_Activities_and_Molecular_Diversity_Analysis_of_Antagonists/4542694
<p>The fungus <i>Trichoderma</i> is a teleomorph of the <i>Hypocrea</i>
genus and associated with biological control of plant diseases. The
microscopic, biochemical, and molecular characterization of <i>Trichoderma </i>was carried out and evaluated for in vitro antagonistic activity against the fungal pathogen <i>Sclerotium rolfsii</i> causing stem rot disease in groundnut. In total, 11 isolates of <i>Trichoderma</i> were examined for antagonism at 6 and 12 days after inoculation (DAI). Out of 11, <i>T. virens </i>NBAII Tvs12 evidenced the highest (87.91%) growth inhibition of the test pathogen followed by <i>T. koningii</i> MTCC 796 (67.03%), <i>T. viride </i>NBAII Tv23 (63.74%), and <i>T. harzianum </i>NBAII
Th1 (60.44%). Strong mycoparasitism was observed in the best antagonist
Tvs12 strain during 6-12 DAI. The specific activity of cell
wall-degrading enzymes - chitinase and β-1,3-glucanase - was positively
correlated with growth inhibition of the test pathogen. In total, 18
simple sequence repeat (SSR) polymorphisms were reported to amplify 202
alleles across 11 <i>Trichoderma </i>isolates. The average polymorphism
information content for SSR markers was found to be 0.80. The best
antagonist Tvs 12 was identified with 7 unique SSR alleles amplified by 5
SSR markers. Clustering patterns of 11 <i>Trichoderma </i>strains showed the best antagonist <i>T. virens</i> NBAII Tvs 12 outgrouped with a minimum 3% similarity from the rest of <i>Trichoderma.</i></p>