10.6084/m9.figshare.4519058.v1
Hitoshi Nakayama
Hitoshi
Nakayama
Mariko Mori
Mariko
Mori
Teisuke Takita
Teisuke
Takita
Kiyoshi Yasukawa
Kiyoshi
Yasukawa
Keisuke Tanaka
Keisuke
Tanaka
Shunji Hattori
Shunji
Hattori
Hideaki Aikawa
Hideaki
Aikawa
Osamu Hasegawa
Osamu
Hasegawa
Takashi Okamura
Takashi
Okamura
Kentarou Takegami
Kentarou
Takegami
Shogo Motokawa
Shogo
Motokawa
Masakazu Kuwahara
Masakazu
Kuwahara
Kenichi Amano
Kenichi
Amano
Development of immersion vaccine for bacterial cold-water disease in ayu <i>Plecoglossus altivelis</i>
Taylor & Francis Group
2017
bacterial cold-water disease
Flavobacterium psychrophilum
ayu Plecoglossus altivelis
collagenase
immersion vaccine
2017-01-04 20:03:52
Presentation
https://tandf.figshare.com/articles/presentation/Development_of_immersion_vaccine_for_bacterial_cold-water_disease_in_ayu_i_Plecoglossus_altivelis_i_/4519058
<p><i>Flavobacterium psychrophilum</i> (<i>F. psychrophilum</i>) is the causative agent of bacterial cold-water disease (BCWD) that occurs in ayu <i>Plecoglossus altivelis</i>. Formalin-killed cell of <i>F. psychrophilum</i> has long been studied as an immersion vaccine for BCWD. In this study, we explored the possibility of <i>F. psychrophilum</i> collagenase (fpcol) for use as the immersion vaccine. BCWD convalescent ayu sera contained specific IgM antibodies against somatic <i>F. psychrophilum</i> and fpcol, meaning that fpcol is a promising antigen for the vaccine development. The recombinant fpcol was successfully expressed in <i>Escherichia coli</i> and <i>Brevibacillus chosinensis</i> (<i>B. chosinensis</i>). The culture supernatant of the <i>B. chosinensis</i> was used as an immersion vaccine solution. The vaccinated ayu were then challenged by soaking into <i>F. psychrophilum</i> culture. In two experimental groups, the relative percentages of survivals were 63 and 38%, respectively, suggesting that fpcol is promising as the immersion vaccine for ayu-BCWD.</p>