%0 Journal Article
%A Ogata, Alana
F.
%A Edgar, Joshua M.
%A Majumdar, Sudipta
%A Briggs, Jeffrey S.
%A Patterson, Shae V.
%A Tan, Ming X.
%A Kudlacek, Stephan T.
%A Schneider, Christine A.
%A Weiss, Gregory A.
%A Penner, Reginald M.
%D 2016
%T Virus-Enabled Biosensor for Human Serum Albumin
%U https://acs.figshare.com/articles/journal_contribution/Virus-Enabled_Biosensor_for_Human_Serum_Albumin/4509536
%R 10.1021/acs.analchem.6b04840.s001
%2 https://ndownloader.figshare.com/files/7293110
%K two-electrode electrochemical biosensor
%K 5 μ M
%K HSA
%K virus-PEDOT films show
%K COV
%K M 13 virus particles
%K 50 μ L
%K Z re
%K Human Serum Albumin
%K Z re signal
%X The label-free detection
of human serum albumin (HSA) in aqueous
buffer is demonstrated using a simple, monolithic, two-electrode electrochemical
biosensor. In this device, both millimeter-scale electrodes are coated
with a thin layer of a composite containing M13 virus particles and
the electronically conductive polymer poly(3,4-ethylenedioxy thiophene)
or PEDOT. These virus particles, engineered to selectively bind HSA,
serve as receptors in this biosensor. The resistance component of
the electrical impedance, Zre, measured
between these two electrodes provides electrical transduction of HSA
binding to the virus-PEDOT film. The analysis of sample volumes as
small as 50 μL is made possible using a microfluidic cell. Upon
exposure to HSA, virus-PEDOT films show a prompt increase in Zre within 5 s and a stable Zre signal within 15 min. HSA concentrations in the range
from 100 nM to 5 μM are detectable. Sensor-to-sensor reproducibility
of the HSA measurement is characterized by a coefficient-of-variance
(COV) ranging from 2% to 8% across this entire concentration range.
In addition, virus-PEDOT sensors successfully detected HSA in synthetic
urine solutions.
%I ACS Publications