10.1371/journal.pgen.1002057.g003 Peter C. Stirling Peter C. Stirling Michelle S. Bloom Michelle S. Bloom Tejomayee Solanki-Patil Tejomayee Solanki-Patil Stephanie Smith Stephanie Smith Payal Sipahimalani Payal Sipahimalani Zhijian Li Zhijian Li Megan Kofoed Megan Kofoed Shay Ben-Aroya Shay Ben-Aroya Kyungjae Myung Kyungjae Myung Philip Hieter Philip Hieter A screen for altered telomeres among poorly characterized essential CIN genes identifies components of ASTRA/TTT. Public Library of Science 2011 altered telomeres poorly characterized cin genes identifies components 2011-04-28 02:43:20 Figure https://plos.figshare.com/articles/figure/_A_screen_for_altered_telomeres_among_poorly_characterized_essential_CIN_genes_identifies_components_of_ASTRA_TTT_/449800 <p>(A) Southern blots of telomere length alterations in indicated mutants. 11 strains are shown, other strains screened had WT telomere length (data not shown). Telomeres are bright bands under 1.5 kb, control bands are seen at the top and bottom of the gel. Triangles indicate short (filled) or long (open) telomeres. (B) Conservation of ASTRA components from yeast to humans. (C) Temperature-sensitive growth of TTT subunit and <i>ASA1</i> mutant alleles in spots of 10-fold serially diluted yeast cultures. (D) Southern blot of TTT-Asa1 telomere length as in (A). (E) Western analysis of 2xFLAG-Tel1p in the TTT-Asa1 ts-alleles including α-Pgk1p blots as a loading control.</p>