10.1371/journal.pgen.1002057.g003
Peter C. Stirling
Peter
C. Stirling
Michelle S. Bloom
Michelle
S. Bloom
Tejomayee Solanki-Patil
Tejomayee
Solanki-Patil
Stephanie Smith
Stephanie
Smith
Payal Sipahimalani
Payal
Sipahimalani
Zhijian Li
Zhijian
Li
Megan Kofoed
Megan
Kofoed
Shay Ben-Aroya
Shay
Ben-Aroya
Kyungjae Myung
Kyungjae
Myung
Philip Hieter
Philip
Hieter
A screen for altered telomeres among poorly characterized essential CIN genes identifies components of ASTRA/TTT.
Public Library of Science
2011
altered
telomeres
poorly
characterized
cin
genes
identifies
components
2011-04-28 02:43:20
Figure
https://plos.figshare.com/articles/figure/_A_screen_for_altered_telomeres_among_poorly_characterized_essential_CIN_genes_identifies_components_of_ASTRA_TTT_/449800
<p>(A) Southern blots of telomere length alterations in indicated mutants. 11 strains are shown, other strains screened had WT telomere length (data not shown). Telomeres are bright bands under 1.5 kb, control bands are seen at the top and bottom of the gel. Triangles indicate short (filled) or long (open) telomeres. (B) Conservation of ASTRA components from yeast to humans. (C) Temperature-sensitive growth of TTT subunit and <i>ASA1</i> mutant alleles in spots of 10-fold serially diluted yeast cultures. (D) Southern blot of TTT-Asa1 telomere length as in (A). (E) Western analysis of 2xFLAG-Tel1p in the TTT-Asa1 ts-alleles including α-Pgk1p blots as a loading control.</p>