10.6084/m9.figshare.4491611.v1
Ilse Gosens
Ilse
Gosens
Flemming R. Cassee
Flemming R.
Cassee
Michela Zanella
Michela
Zanella
Laura Manodori
Laura
Manodori
Andrea Brunelli
Andrea
Brunelli
Anna Luisa Costa
Anna Luisa
Costa
Bas G. H. Bokkers
Bas G. H.
Bokkers
Wim H. de Jong
Wim
H. de Jong
David Brown
David
Brown
Danail Hristozov
Danail
Hristozov
Vicki Stone
Vicki
Stone
Organ burden and pulmonary toxicity of nano-sized copper (II) oxide particles after short-term inhalation exposure
Taylor & Francis Group
2016
CuO NPs
histopathological changes
exposure time
dose levels
Organ burden
inhalation route
risk assessment
MMAD
nano-sized copper
dose-dependent toxicity
1.5 μ m
3- week post-exposure period
13.2
6 h-concentration equivalents
dose-dependent lung inflammation
exposure route
copper oxide nanoparticles
24 h
exposure concentration
1.5 μ
particle size
hazard data
mg
inhalation exposure Introduction
recovery period
oxide particles
Histopathological examinations
II
agglomerated CuO NPs
2016-12-22 16:20:37
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Organ_burden_and_pulmonary_toxicity_of_nano-sized_copper_II_oxide_particles_after_short-term_inhalation_exposure/4491611
<p><b>Introduction</b>: Increased use of nanomaterials has raised concerns about the potential for undesirable human health and environmental effects. Releases into the air may occur and, therefore, the inhalation route is of specific interest. Here we tested copper oxide nanoparticles (CuO NPs) after repeated inhalation as hazard data for this material and exposure route is currently lacking for risk assessment.</p> <p><b>Methods</b>: Rats were exposed nose-only to a single exposure concentration and by varying the exposure time, different dose levels were obtained (C × T protocol). The dose is expressed as 6 h-concentration equivalents of 0, 0.6, 2.4, 3.3, 6.3, and 13.2 mg/m<sup>3</sup> CuO NPs, with a primary particle size of 10 9.2–14 nm and an MMAD of 1.5 μm.</p> <p><b>Results</b>: Twenty-four hours after a 5-d exposure, dose-dependent lung inflammation and cytotoxicity were observed. Histopathological examinations indicated alveolitis, bronchiolitis, vacuolation of the respiratory epithelium, and emphysema in the lung starting at 2.4 mg/m<sup>3</sup>. After a recovery period of 22 d, limited inflammation was still observed, but only at the highest dose of 13.2 mg/m<sup>3</sup>. The olfactory epithelium in the nose degenerated 24 h after exposure to 6.3 and 13.2 mg/m<sup>3</sup>, but this was restored after 22 d. No histopathological changes were detected in the brain, olfactory bulb, spleen, kidney and liver.</p> <p><b>Conclusion</b>: A 5-d, 6-h/day exposure equivalent to an aerosol of agglomerated CuO NPs resulted in a dose-dependent toxicity in rats, which almost completely resolved during a 3-week post-exposure period.</p>