10.6084/m9.figshare.c.3646397_D3.v1 M. Brahimi-Horn M. Brahimi-Horn Sandy Giuliano Sandy Giuliano Estelle Saland Estelle Saland Sandra Lacas-Gervais Sandra Lacas-Gervais Tatiana Sheiko Tatiana Sheiko Joffrey Pelletier Joffrey Pelletier Isabelle Bourget Isabelle Bourget Frédéric Bost Frédéric Bost Chloé Féral Chloé Féral Etienne Boulter Etienne Boulter Michel Tauc Michel Tauc Mircea Ivan Mircea Ivan Barbara Garmy-Susini Barbara Garmy-Susini Alexandra Popa Alexandra Popa Bernard Mari Bernard Mari Jean-Emmanuel Sarry Jean-Emmanuel Sarry William Craigen William Craigen Jacques Pouysségur Jacques Pouysségur Nathalie Mazure Nathalie Mazure Additional file 18: Figure S10. of Knockout of Vdac1 activates hypoxia-inducible factor through reactive oxygen species generation and induces tumor growth by promoting metabolic reprogramming and inflammation Springer Nature 2015 Wt RAS MEF Soft agar assay RASV FCCP OCR Glu 10  mM reactive oxygen species generation oxygen consumption rate ECAR Oligo 1 μ M Nx 1 μ X μ m extracellular acidification rate WT RAS MEF Representative phase contrast photographs clonogenic cell survival assay Seahorse XF bioenergetic system Vdac 1 activates hypoxia-inducible factor 2015-08-26 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_18_Figure_S10_of_Knockout_of_Vdac1_activates_hypoxia-inducible_factor_through_reactive_oxygen_species_generation_and_induces_tumor_growth_by_promoting_metabolic_reprogramming_and_inflammation/4475129 Characterization of RASV12 transformed MEF expressing (WT RAS MEF) or not (Vdac1 −/− RAS MEF) Vdac1. (A) Characterization of the growth of Wt and Vdac1 −/− RAS MEF incubated in Nx or Hx for the indicated number of days. The mean ± SEM is representative of four independent experiments carried out in duplicate. A p < 0.00001 shows significant difference from the normoxia for Vdac1 −/− RAS MEF. (B) Representative phase contrast photographs of Wt and Vdac1 −/− RAS MEF incubated in Nx for 72 h. Scale bars represent X μm. (C) Relative migration of Wt and Vdac1 −/− RAS MEF in Nx as evaluated in a xCELLigence system. The mean ± SEM is representative of two independent experiments carried out in quadruplicate. (D) The extracellular acidification rate (ECAR) in Nx of Wt and Vdac1 −/− RAS MEF was evaluated with a Seahorse XF bioenergetic system. Glucose (Glu 10 mM) and oligomycin (Oligo 1 μM) were injected at the indicated times. (E) The oxygen consumption rate (OCR) in Nx for Wt and Vdac1 −/− RAS MEF was measured in real time with a Seahorse XF. Glucose (Glu 10 mM), oligomycin (Oligo 1 μM), carbonilcyanide p-triflouromethoxyphenylhydrazone (FCCP 1 μM), and Rotenone/Antimycine A (Rot/AA, 1 μM/1 μM) were injected at the indicated times. The mean ± SEM is representative of three independent experiments carried out in quadruplicate. (F) Radioresistance of Wt and Vdac1 −/− RAS MEF cultured for 24 h in Nx or Hx and treated with the indicated dose of radiation. Cell growth was then evaluated with a clonogenic cell survival assay. X-axis: dose of X-radiation (Gy). Y-axis: surviving fraction. The mean ± SEM is representative of two independent experiments carried out in duplicate. (G) Soft agar assay of Wt and Vdac1 −/− RAS MEF. (H) Tumor weight of Wt (Wt RAS MEF) and Vdac1 −/− RAS MEF-derived tumors (Vdac1 −/− RAS MEF).