10.6084/m9.figshare.c.3646397_D3.v1
M. Brahimi-Horn
M.
Brahimi-Horn
Sandy Giuliano
Sandy
Giuliano
Estelle Saland
Estelle
Saland
Sandra Lacas-Gervais
Sandra
Lacas-Gervais
Tatiana Sheiko
Tatiana
Sheiko
Joffrey Pelletier
Joffrey
Pelletier
Isabelle Bourget
Isabelle
Bourget
Frédéric Bost
Frédéric
Bost
Chloé Féral
Chloé
Féral
Etienne Boulter
Etienne
Boulter
Michel Tauc
Michel
Tauc
Mircea Ivan
Mircea
Ivan
Barbara Garmy-Susini
Barbara
Garmy-Susini
Alexandra Popa
Alexandra
Popa
Bernard Mari
Bernard
Mari
Jean-Emmanuel Sarry
Jean-Emmanuel
Sarry
William Craigen
William
Craigen
Jacques Pouysségur
Jacques
Pouysségur
Nathalie Mazure
Nathalie
Mazure
Additional file 18: Figure S10. of Knockout of Vdac1 activates hypoxia-inducible factor through reactive oxygen species generation and induces tumor growth by promoting metabolic reprogramming and inflammation
Springer Nature
2015
Wt RAS MEF
Soft agar assay
RASV
FCCP
OCR
Glu 10 mM
reactive oxygen species generation
oxygen consumption rate
ECAR
Oligo 1 μ M
Nx
1 μ
X μ m
extracellular acidification rate
WT RAS MEF
Representative phase contrast photographs
clonogenic cell survival assay
Seahorse XF bioenergetic system
Vdac 1 activates hypoxia-inducible factor
2015-08-26 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_18_Figure_S10_of_Knockout_of_Vdac1_activates_hypoxia-inducible_factor_through_reactive_oxygen_species_generation_and_induces_tumor_growth_by_promoting_metabolic_reprogramming_and_inflammation/4475129
Characterization of RASV12 transformed MEF expressing (WT RAS MEF) or not (Vdac1 −/− RAS MEF) Vdac1. (A) Characterization of the growth of Wt and Vdac1 −/− RAS MEF incubated in Nx or Hx for the indicated number of days. The mean ± SEM is representative of four independent experiments carried out in duplicate. A p < 0.00001 shows significant difference from the normoxia for Vdac1 −/− RAS MEF. (B) Representative phase contrast photographs of Wt and Vdac1 −/− RAS MEF incubated in Nx for 72 h. Scale bars represent X μm. (C) Relative migration of Wt and Vdac1 −/− RAS MEF in Nx as evaluated in a xCELLigence system. The mean ± SEM is representative of two independent experiments carried out in quadruplicate. (D) The extracellular acidification rate (ECAR) in Nx of Wt and Vdac1 −/− RAS MEF was evaluated with a Seahorse XF bioenergetic system. Glucose (Glu 10 mM) and oligomycin (Oligo 1 μM) were injected at the indicated times. (E) The oxygen consumption rate (OCR) in Nx for Wt and Vdac1 −/− RAS MEF was measured in real time with a Seahorse XF. Glucose (Glu 10 mM), oligomycin (Oligo 1 μM), carbonilcyanide p-triflouromethoxyphenylhydrazone (FCCP 1 μM), and Rotenone/Antimycine A (Rot/AA, 1 μM/1 μM) were injected at the indicated times. The mean ± SEM is representative of three independent experiments carried out in quadruplicate. (F) Radioresistance of Wt and Vdac1 −/− RAS MEF cultured for 24 h in Nx or Hx and treated with the indicated dose of radiation. Cell growth was then evaluated with a clonogenic cell survival assay. X-axis: dose of X-radiation (Gy). Y-axis: surviving fraction. The mean ± SEM is representative of two independent experiments carried out in duplicate. (G) Soft agar assay of Wt and Vdac1 −/− RAS MEF. (H) Tumor weight of Wt (Wt RAS MEF) and Vdac1 −/− RAS MEF-derived tumors (Vdac1 −/− RAS MEF).