Additional file 5: Figure S4. of Errors in RNA-Seq quantification affect genes of relevance to human disease Christelle Robert Mick Watson 10.6084/m9.figshare.c.3643154_D7.v1 https://springernature.figshare.com/articles/figure/Additional_file_5_Figure_S4_of_Errors_in_RNA-Seq_quantification_affect_genes_of_relevance_to_human_disease/4464488 General characteristics of third problematic group. Boxplots comparing the length of the shortest exon, the length of the longest exon, the mean exon length, the total number of exons, the transcript length, transcript percentage GC, the number of reads overlapping from the STAR alignment and the number of reads overlapping the TopHat alignment for a group of genes where HTSeq underestimates, Cufflinks and Sailfish are accurate but the use of the --multi-read-correct parameter in Cuffinks results in underestimation. (JPEG 575 kb) 2015-09-03 05:00:00 Cuffinks results multi-read-correct parameter exon length JPEG 575 kb disease General characteristics RNA-Seq quantification STAR alignment TopHat alignment transcript percentage GC transcript length gene