Chen, Shilong Luo, Guifeng Yang, Zhou Lin, Shuncheng Chen, Shaoying Wang, Song Goraya, Mohsan Chi, Xiaojuan Zeng, Xiancheng Chen, Ji-Long MOESM7 of Avian Tembusu virus infection effectively triggers host innate immune response through MDA5 and TLR3-dependent signaling pathways Additional file 7 IRF3, IRF7 and NF-κB activation are required for efficient expression of IFN induced by ATMUV. A and B. IRF3, IRF7 knockdown 293T cell lines or luciferase control were infected with or without ATMUV for 36 h. RT-PCR were performed to measure the interference efficiency of IRF3, IRF7 and the production of IFN-β, IL-28A/B and IL-29. C. 293T cells were treated with BAY11-7082 (5 µM) or DMSO for 3 h, followed by ATMUV infection for 36 h. The cells were harvested and the mRNA levels of IFN-β, IL-28A/B and IL-29 were analyzed by RT-PCR. 36 h;293 T cells;IFN;Avian Tembusu virus infection;RT-PCR;MDA;TLR;NF -κB activation;IRF;IL -29. C;MOESM;-28A;ATMUV;DMSO 2016-07-22
    https://springernature.figshare.com/articles/journal_contribution/MOESM7_of_Avian_Tembusu_virus_infection_effectively_triggers_host_innate_immune_response_through_MDA5_and_TLR3-dependent_signaling_pathways/4464083
10.6084/m9.figshare.c.3643004_D8.v1