TY - DATA T1 - Additional file 1: Figure S1. of Apremilast, a novel phosphodiesterase 4 (PDE4) inhibitor, regulates inflammation through multiple cAMP downstream effectors PY - 2015/09/15 AU - Miguel Perez-Aso AU - M. Montesinos AU - Aránzazu Mediero AU - Tuere Wilder AU - Peter Schafer AU - Bruce Cronstein UR - https://springernature.figshare.com/articles/figure/Additional_file_1_Figure_S1_of_Apremilast_a_novel_phosphodiesterase_4_PDE4_inhibitor_regulates_inflammation_through_multiple_cAMP_downstream_effectors/4456835 DO - 10.6084/m9.figshare.c.3640835_D2.v1 L4 - https://ndownloader.figshare.com/files/7186088 KW - TIFF 170 kb KW - CGS 21680 KW - intracellular cAMP levels KW - novel phosphodiesterase 4 KW - 2AR activation KW - effectors Adenosine KW - nM KW - Raw 264.7 cells KW - 1 μ M KW - concentration KW - element binding protein KW - apremilast KW - LPS KW - 20 minutes KW - 2A receptor KW - PDE KW - additively increase KW - CGS 21680 1μ M 15 minutes N2 - Adenosine A2A receptor (A2AR) activation and apremilast do not additively increase responsive element binding protein (cAMP). Raw 264.7 cells were incubated with cumulative concentrations of apremilast (6 nM to 1 μM), apremilast + CGS21680 1μM 15 minutes before apremilast, or cumulative concentrations of CGS21680 alone (6 nM to 1 μM), followed by treatment with lipopolysaccharide (LPS) 1 μM for 20 minutes. Then, intracellular cAMP levels were measured as described under “Materials and methods”. Data represent means ± standard error of the mean of at least three independent experiments. (TIFF 170 kb) ER -