10.6084/m9.figshare.c.3639167_D4.v1
Sean Monaghan
Sean
Monaghan
Kim Thompson
Kim
Thompson
James Bron
James
Bron
Sven Bergmann
Sven
Bergmann
Tae Jung
Tae
Jung
Takashi Aoki
Takashi
Aoki
K. Muir
K.
Muir
Malte Dauber
Malte
Dauber
Sven Reiche
Sven
Reiche
Diana Chee
Diana
Chee
Shin Chong
Shin
Chong
Jing Chen
Jing
Chen
Alexandra Adams
Alexandra
Adams
MOESM3 of Expression of immunogenic structural proteins of cyprinid herpesvirus 3 in vitro assessed using immunofluorescence
Springer Nature
2016
MAb 20 F 10
salmon anaemia virus
CyHV -3
image analysis macro
quantification parameters utilised
20 F 10.
confocal microscopy fluorescence
PBS
time post infection
KF
MOESM
ISA
MAb 20 F 10.
Envelope glycoprotein MAb
image analysis
cyprinid herpesvirus 3
CCB cells
2016-01-08 05:00:00
Figure
https://springernature.figshare.com/articles/figure/MOESM3_of_Expression_of_immunogenic_structural_proteins_of_cyprinid_herpesvirus_3_in_vitro_assessed_using_immunofluorescence/4451363
Additional file 3. Transformation of confocal microscopy fluorescence for image analysis in CyHV-3 infected KF-1 and CCB cells screened with MAbs 10A9 and 20F10. (A) Example for CyHV-3 infected CCB cells after 3 and 7 dpi screened with MAb 20F10. The parameter converted for image analysis is indicated above each column. Top row = confocal micrographs, bottom row = image utilised for image analysis subsequent to transformation (B) Images of quantification parameters utilised by the image analysis macro. The parameter measured for image analysis is above the micrographs and above this is the cell line used. Time post infection is indicated on the right of each row as well as the MAb used for screening. MAb 10A9 = Envelope glycoprotein MAb; MAb 20F10 = Capsid-associated MAb; ISA MAb = Irrelevant MAb detecting infectious salmon anaemia virus; PBS = phosphate buffered saline.