10.6084/m9.figshare.c.3639167_D3.v1 Sean Monaghan Sean Monaghan Kim Thompson Kim Thompson James Bron James Bron Sven Bergmann Sven Bergmann Tae Jung Tae Jung Takashi Aoki Takashi Aoki K. Muir K. Muir Malte Dauber Malte Dauber Sven Reiche Sven Reiche Diana Chee Diana Chee Shin Chong Shin Chong Jing Chen Jing Chen Alexandra Adams Alexandra Adams MOESM2 of Expression of immunogenic structural proteins of cyprinid herpesvirus 3 in vitro assessed using immunofluorescence Springer Nature 2016 Red line MAb 20 F 10 DAPI fluorescence MAbs 20 F 10 MAb 10 MOESM 2 Envelope glycoprotein antigen FITC infection DAPI stain cell cultures 0 hpi 9 recognising Koi herpesvirus antigens cyprinid herpesvirus 3 2. Graphical representation Âą SE CCB cells microtitre plates 2016-01-08 05:00:00 Figure https://springernature.figshare.com/articles/figure/MOESM2_of_Expression_of_immunogenic_structural_proteins_of_cyprinid_herpesvirus_3_in_vitro_assessed_using_immunofluorescence/4451360 Additional file 2. Graphical representation of fluorescence from MAbs 20F10 and 10A9 recognising Koi herpesvirus antigens over the course of infection in CCB cells in 96 well microtitre plates. Graphs are of cells analysed during the first day of infection. (A) DAPI fluorescence of cells; (B) Difference of relative FITC to DAPI stain from blank wells at 0 hpi. Red line = MAb 20F10 (Capsid-associated antigen); Blue line = MAb 10A9 (Envelope glycoprotein antigen). Mean Âą SE (n = 4 individual cell cultures).