Additional file 2: Figure S2. of A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica
Knut Rennert
Peter Otto
Harald Funke
Otmar Huber
Herbert Tomaso
Alexander Mosig
10.6084/m9.figshare.c.3637682_D2.v1
https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S2_of_A_human_macrophage_hepatocyte_co-culture_model_for_comparative_studies_of_infection_and_replication_of_Francisella_tularensis_LVS_strain_and_subspecies_holarctica_and_mediasiatica/4445336
Detection of intracellular LPS in macrophage / hepatocyte co-cultures infected with LVS (open bars), spp. holarctica (grey filled bars) or spp. mediasiatica (black filled bars) and untreated control (hatched bars). A) Different amounts of macrophages in the co-culture were tested (6, 12 and 22 % of macrophages on total cell count). Flow cytometric detection of intracellular LPS in macrophages (MFI mean fluorescence intensity); B-D) percentage of remaining detectable macrophages after infection of the co-cultures with B) 6 % macrophages/94 % hepatocytes, C) 12 % macrophages/ 88 % hepatocytes and D) 22 % macrophages/ 88 % hepatocytes 72 h post infection. (TIF 32735 kb)
2016-01-06 05:00:00
Francisella tularensis
Human macrophage
Hepatocyte
Co-culture
Liver