Additional file 2: Figure S2. of A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica Knut Rennert Peter Otto Harald Funke Otmar Huber Herbert Tomaso Alexander Mosig 10.6084/m9.figshare.c.3637682_D2.v1 https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S2_of_A_human_macrophage_hepatocyte_co-culture_model_for_comparative_studies_of_infection_and_replication_of_Francisella_tularensis_LVS_strain_and_subspecies_holarctica_and_mediasiatica/4445336 Detection of intracellular LPS in macrophage / hepatocyte co-cultures infected with LVS (open bars), spp. holarctica (grey filled bars) or spp. mediasiatica (black filled bars) and untreated control (hatched bars). A) Different amounts of macrophages in the co-culture were tested (6, 12 and 22 % of macrophages on total cell count). Flow cytometric detection of intracellular LPS in macrophages (MFI mean fluorescence intensity); B-D) percentage of remaining detectable macrophages after infection of the co-cultures with B) 6 % macrophages/94 % hepatocytes, C) 12 % macrophages/ 88 % hepatocytes and D) 22 % macrophages/ 88 % hepatocytes 72 h post infection. (TIF 32735 kb) 2016-01-06 05:00:00 Francisella tularensis Human macrophage Hepatocyte Co-culture Liver