Additional file 7: of Cigarette smoke alters the ability of human dendritic cells to promote anti-Streptococcus pneumoniae Th17 response Olivier Le Rouzic Bachirou Koné Jerome Kluza Philippe Marchetti Florence Hennegrave Cécile Olivier Gwenola Kervoaze Eva Vilain Clémence Mordacq Nicolas Just Thierry Perez Nathalie Bautin Muriel Pichavant Philippe Gosset 10.6084/m9.figshare.c.3637556_D6.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_7_of_Cigarette_smoke_alters_the_ability_of_human_dendritic_cells_to_promote_anti-Streptococcus_pneumoniae_Th17_response/4445021 In vitro exposure to rotenone (Rot) or antimycin A (AmA) inhibits the phenotype of monocyte-derived dendritic cells (MDDC) activated by S.pneumoniae (Sp). Expression of HLA-DR (a), CD80 (b), CD86 (c), CD40 (d) and CD54 (e) were evaluated by flow cytometry in MDDC exposed to rotenone or antimycin A and then activated or not by S.pneumoniae for 24 h. Data represent mean ± S.E.M. of 6 experitments. There were no statistical differences between groups. (PDF 50 kb) 2016-07-26 05:00:00 Chronic obstructive pulmonary disease Dendritic cells Streptococcus pneumoniae Smoking Interleukin-1β Interleukin-23 Th17 cytokines