10.6084/m9.figshare.c.3637034_D1.v1
Melissa Castillo-Lizardo
Melissa
Castillo-Lizardo
Isabel Aragón
Isabel
Aragón
Vivian Carvajal
Vivian
Carvajal
Isabel Matas
Isabel
Matas
María Pérez-Bueno
María
Pérez-Bueno
María-Trinidad Gallegos
María-Trinidad
Gallegos
Matilde Barón
Matilde
Barón
Cayo Ramos
Cayo
Ramos
Additional file 2: Figure S2. of Contribution of the non-effector members of the HrpL regulon, iaaL and matE, to the virulence of Pseudomonas syringae pv. tomato DC3000 in tomato plants
Springer Nature
2015
tomato DC 3000
hrp box motifs
TIFF 3064 kb
sequence
matE
primers R 161
tomato DC 3000.
Pseudomonas syringae pv
gene
iaaL
dideoxy sequencing reactions
site
ORFS
syringae pv
RNA
runoff cDNAs
transcription
RACE
primer R 161. Arrows
tomato plants Determination
2015-08-19 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S2_of_Contribution_of_the_non-effector_members_of_the_HrpL_regulon_iaaL_and_matE_to_the_virulence_of_Pseudomonas_syringae_pv_tomato_DC3000_in_tomato_plants/4443437
Determination of the transcription start sites of the iaaL and matE genes in P. syringae pv. tomato DC3000. Total RNA of P. syringae pv. tomato DC3000 grown on minimal media was extracted. (A) For iaaL, runoff cDNAs were generated by extending primers R161 and R121 (Table S1). Lanes A, C, G, and T contain the products of the dideoxy sequencing reactions with using primer R161. Arrows indicate the size of the runoff cDNAs obtained for each primer. (B) Nucleotide sequence of the intergenic region including the 3′ and 5′ ends of the matE and iaaL ORFS, respectively. (C) For matE, the +1 position was determined by 5′RACE and subsequent sequencing. B and C: The coding sequences of both genes are shadowed. The transcription start sites (P iaaL/matE) are marked with a black spot and are shown in bold type; the arrowhead indicates the direction of transcription. The hrp box motifs are shown in gray boxes, and the sequences of the primers used are underlined. (TIFF 3064 kb)