10.6084/m9.figshare.c.3636671_D7.v1 Daria Drobysheva Daria Drobysheva Brittni Smith Brittni Smith Maria McDowell Maria McDowell Katrin Guillen Katrin Guillen Huseyin Ekiz Huseyin Ekiz Bryan Welm Bryan Welm Additional file 5: Figure S5. of Transformation of enriched mammary cell populations with polyomavirus middle T antigen influences tumor subtype and metastatic potential Springer Nature 2015 tumor histology Molecularly basal tumors Western blot quantification SRC protein expression ERK t test tumor groups polyomavirus middle T antigen influences tumor subtype population tumors PyMT activity comparison AKT blot quantification protein expression TIFF 1046 kb β- actin 2015-10-01 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_5_Figure_S5_of_Transformation_of_enriched_mammary_cell_populations_with_polyomavirus_middle_T_antigen_influences_tumor_subtype_and_metastatic_potential/4442267 PyMT activity comparison with tumor histology, molecular subtype, and metastasis. a Western blot quantification of relative ratio of pAKT to AKT, pERK to ERK, and pSRC to SRC protein expression in enriched population tumors segregated based on tumor histology and normalized to β-actin. No significant differences in protein expression were detected among tumor groups (t test, n = number of tumors). b Western blot quantification of relative ratio of pAKT to AKT, pERK to ERK, and pSRC to SRC protein expression in enriched population tumors segregated based on tumor molecular subtype and normalized to β-actin. Molecularly basal tumors exhibited significantly higher levels of pERK/ERK levels than luminal tumors (t test, p value shown, n = number of tumors). c Western blot quantification of relative ratio of pAKT to AKT, pERK to ERK, and pSRC to SRC protein expression in enriched population tumors segregated based on tumor metastasis and normalized to β-actin. No significant differences in protein expression were detected among tumor groups (t test, n = number of tumors). (TIFF 1046 kb)