Menendez-Bravo, Simón Roulet, Julia Sabatini, Martín Comba, Santiago Dunn, Robert Gramajo, Hugo Arabolaza, Ana MOESM1 of High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties Additional file 1: Figure S1. Protein expression in different E. coli RQ1 derivatives strains. A. Western blot analysis of RQ1 derivative strain expressing extra copies of S. coelicolor PCC complex. (1) RQ1/pMB07 induced with 0.1 mM IPTG. (2) RQ1/pMB07/pMB20 not induced. (3) RQ1/pMB07/pMB20 induced with 0.1 mM IPTG. B. Coomasie stained SDS-PAGE of RQ1/pMB07. (1) RQ1/pMB07 not induced. (2) RQ1/pMB07 induced with 0.1 mM IPTG. C. Western blot analysis of RQ1 derivative strain expressing extra copies of papA5. (1) RQ1/pMB07 induced with 0.1 mM IPTG. (2) RQ1/pMB07/pMB04 not induced. (3) RQ1/pMB07/pMB04 induced with 0.1 mM IPTG and 0.2 % l-Ara. D. Western blot analysis of RQ1 derivative strain expressing extra copies of fadD28. (1) RQ1/pMB07 induced with 0.1 mM IPTG. (2) RQ1/pMB07/pMB05 induced with 0.1 mM IPTG and 0.2 % l-Ara. Escherichia coli;Wax production;Metabolic engineering;Fed-batch fermentation;Oleochemicals 2016-10-14
    https://springernature.figshare.com/articles/journal_contribution/MOESM1_of_High_cell_density_production_of_multimethyl-branched_long-chain_esters_in_Escherichia_coli_and_determination_of_their_physicochemical_properties/4440272
10.6084/m9.figshare.c.3636035_D2.v1