Additional file 1: Figure S1. of Cytosolic phospholipase A2 contributes to innate immune defense against Candida albicans lung infection
Sabarirajan Jayaraja
Azzeddine Dakhama
Bogeon Yun
Moumita Ghosh
HeeJung Lee
Elizabeth Redente
Charis Uhlson
Robert Murphy
Christina Leslie
10.6084/m9.figshare.c.3634640_D2.v1
https://springernature.figshare.com/articles/figure/Additional_file_1_Figure_S1_of_Cytosolic_phospholipase_A2_contributes_to_innate_immune_defense_against_Candida_albicans_lung_infection/4435958
Flow cytometry gating strategy for cell identification in lung digests from cPLA2α+/+ (WT) and cPLA2α−/− (KO) mice challenged with C. albicans for 24 h. Cells were isolated from enzymatically digested mouse lungs, and after exclusion of doublets and debris, immune cells were identified by CD45 staining. A sequential gating strategy was used to identify populations expressing specific markers: a alveolar macrophages (AM) (CD45+ CD24− CD11b− SiglecF+), (b) tissue macrophages (TM) (CD45+ CD24− CD11b+), (c) neutrophils (PMN) (CD45+ CD11b+ Ly6G+) and (d) CD11b+ dendritic cells (CD11b+ DCs) (CD45+ MHCII+ CD11c+ CD11b+). (TIF 954 kb)
2016-08-08 05:00:00
Cytosolic phospholipase A2
Prostaglandins
Leukotrienes
Neutrophils
Macrophages
Inflammation
Candida albicans