10.6084/m9.figshare.c.3634640_D2.v1
Sabarirajan Jayaraja
Sabarirajan
Jayaraja
Azzeddine Dakhama
Azzeddine
Dakhama
Bogeon Yun
Bogeon
Yun
Moumita Ghosh
Moumita
Ghosh
HeeJung Lee
HeeJung
Lee
Elizabeth Redente
Elizabeth
Redente
Charis Uhlson
Charis
Uhlson
Robert Murphy
Robert
Murphy
Christina Leslie
Christina
Leslie
Additional file 1: Figure S1. of Cytosolic phospholipase A2 contributes to innate immune defense against Candida albicans lung infection
Springer Nature
2016
Cytosolic phospholipase A2
Prostaglandins
Leukotrienes
Neutrophils
Macrophages
Inflammation
Candida albicans
2016-08-08 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_1_Figure_S1_of_Cytosolic_phospholipase_A2_contributes_to_innate_immune_defense_against_Candida_albicans_lung_infection/4435958
Flow cytometry gating strategy for cell identification in lung digests from cPLA2α+/+ (WT) and cPLA2α−/− (KO) mice challenged with C. albicans for 24 h. Cells were isolated from enzymatically digested mouse lungs, and after exclusion of doublets and debris, immune cells were identified by CD45 staining. A sequential gating strategy was used to identify populations expressing specific markers: a alveolar macrophages (AM) (CD45+ CD24− CD11b− SiglecF+), (b) tissue macrophages (TM) (CD45+ CD24− CD11b+), (c) neutrophils (PMN) (CD45+ CD11b+ Ly6G+) and (d) CD11b+ dendritic cells (CD11b+ DCs) (CD45+ MHCII+ CD11c+ CD11b+). (TIF 954 kb)