Chemical profiling of the tuber of <i>Stephania cambodica</i> Gagnep. (Menispermaceae) and analytical control by UHPLC-DAD Chhavarath Dary Sok-Siya Bun Gaëtan Herbette Fathi Mabrouki Hot Bun Sothea Kim Florian Jabbour Sovanmoly Hul Béatrice Baghdikian Evelyne Ollivier 10.6084/m9.figshare.4434941.v1 https://tandf.figshare.com/articles/journal_contribution/Chemical_profiling_of_the_tuber_of_i_Stephania_cambodica_i_Gagnep_Menispermaceae_and_analytical_control_by_UHPLC-DAD/4434941 <p>A new aporphine glycoside (<b>1</b>), named ‘angkorwatine’, and eight known alkaloids: oblongine (<b>2</b>), stepharine (<b>3</b>), asimilobine-β-d-glucopyranoside (<b>4</b>), isocorydine (<b>5</b>), tetrahydropalmatine (THP) (<b>6</b>), jatrorrhizine (<b>7</b>), palmatine (PAL) (<b>8</b>), and roemerine (ROE) (<b>9</b>) were simultaneously isolated from the tuber of <i>Stephania cambodica</i>. The development and validation of UHPLC-DAD method was carried out for the quantification of marker compounds (PAL, ROE, THP) of <i>S. cambodica</i>. In addition to good selectivity and linearity (<i>r</i><sup>2</sup> > 0.997), trueness, precision, and accuracy of the method did not exceed the acceptance limit of ±10% for ROE, THP and ±20% for PAL. Consequently, this method is able to provide accurate results between 1.39–4.18 μg/mL, 2.01–30.72 μg/mL, and 4.29–64.42 μg/mL for PAL, ROE, and THP, respectively. This study shows that the validated UHPLC method is a rapid, innovative and effective analytical approach to control quality of tubers of <i>S. cambodica</i> and to regulate the usage of this plant in traditional medicine.</p> 2016-12-15 11:41:33 Accuracy profile alkaloid method validation palmatine quantification roemerine tetrahydropalmatine