10.6084/m9.figshare.c.3632561_D3.v1 Holli Loomans Holli Loomans Shanna Arnold Shanna Arnold Laura Quast Laura Quast Claudia Andl Claudia Andl Additional file 2: Figure S2. of Esophageal squamous cell carcinoma invasion is inhibited by Activin A in ACVRIB-positive cells Springer Nature 2016 Dysplasia Esophageal cell invasion Cell signaling Fibronectin Podoplanin Angiogenesis 2016-11-09 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S2_of_Esophageal_squamous_cell_carcinoma_invasion_is_inhibited_by_Activin_A_in_ACVRIB-positive_cells/4428701 “Overexpression of Activin A, validated by ELISA, was persistent and did not affect fibroblast contractility”; validation of assays by ELISA and cell contractility assay. (a) Overexpression of Activin A was validated following each retrovirus transduction. Levels of secreted Activin A protein, measured in conditioned media, were significantly higher in Act A compared to parent and empty vector control, ECdnT, TE-2, and TE-11 cells in 2D monolayer. Overexpression of Activin A was validated throughout the 17-day organotypic culture with ECdnT (b), TE-2 (c), and TE-11 (d). Fibro-ActA had sustained increased expression of Activin A during this time period. (e) Parent, empty, and Fibro-ActA had comparable ability to contract collagen, indicating that overexpression of Activin A alone did not hinder the fibroblasts ability to contract the extracellular matrix. 3-butanedione monoxime (BDM) was used as an assay control. (f) Quantification (percent) of fibroblast matrix contraction from (e). (One-way ANOVA, *p<0.05). (TIF 4375 kb)