Additional file 1: Figure S1A. of 3D culture of Her2+ breast cancer cells promotes AKT to MAPK switching and a loss of therapeutic response Sharath Gangadhara Chris Smith Peter Barrett-Lee Stephen Hiscox 10.6084/m9.figshare.c.3631220_D3.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_Figure_S1A_of_3D_culture_of_Her2_breast_cancer_cells_promotes_AKT_to_MAPK_switching_and_a_loss_of_therapeutic_response/4424606 3D ‘On-Top’ Cell culture method. A modified version of the ‘3D on-top’ assay reported by Lee et al. [17] was used for analysis of cellular growth in 3D culture and optimised for the cell lines under test here. (DOC 41 kb) 2016-06-01 05:00:00 3D culture Her2+ breast cancer MAPK AKT Therapeutic response