10.6084/m9.figshare.c.3629300_D4.v1
Kristine Kines
Kristine
Kines
Mark Sokolowski
Mark
Sokolowski
Dawn deHaro
Dawn
deHaro
Claiborne Christian
Claiborne
Christian
Melody Baddoo
Melody
Baddoo
Madison Smither
Madison
Smither
Victoria Belancio
Victoria
Belancio
Additional file 7: Figure S4. of The endonuclease domain of the LINE-1 ORF2 protein can tolerate multiple mutations
Springer Nature
2016
LINE-1
L1
ORF2
Endonuclease
Mutation
Retrotransposition
Phosphorylation
2016-04-19 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_7_Figure_S4_of_The_endonuclease_domain_of_the_LINE-1_ORF2_protein_can_tolerate_multiple_mutations/4418576
Western blot analysis of ORF2 proteins containing mutations in putative phosphorylation sites. Top panel: Representative western blot analysis of total cell lysates harvested from HeLa cells transfected with the indicated ORF2 putative phosphorylation mutant constructs. ORF2 is the functional protein and ORF2 RT- is a non-functional protein containing a mutation in the reverse transcriptase (D702A) domain. Control lanes indicate cells transfected with an empty vector. Lysates were probed with anti-ÎłH2AX antibodies to detect the phosphorylation of histone H2AX in response to DNA damage, top; and anti-GAPDH to serve as a loading control, bottom. Bottom panel: Western blot quantitation. For each sample, the signal detected for ÎłH2AX was normalized to the signal detected for GAPDH. These relative numbers were expressed as a proportion of the relative number detected from the functional ORF2p. (PNG 263Â kb)