Additional file 4: Figure S3. of Prolonged persistence of a novel replication-defective HIV-1 variant in plasma of a patient on suppressive therapy Samantha Rassler Roberto Ramirez Nadeen Khoury Gail Skowron Gautam Sahu 10.6084/m9.figshare.c.3629195_D2.v1 https://springernature.figshare.com/articles/presentation/Additional_file_4_Figure_S3_of_Prolonged_persistence_of_a_novel_replication-defective_HIV-1_variant_in_plasma_of_a_patient_on_suppressive_therapy/4418240 Amplification of R-gag fragments by RT-nested PCR. Longitudinally-collected vRNA samples (i.e., G1P, G2P, etc.) were used as targets for amplification. (PPT 105 kb) 2016-09-21 05:00:00 HIV-1 Antiretroviral therapy Residual plasma viremia 5′-major splice donor motif Novel mutation