10.6084/m9.figshare.c.3626432_D7.v1
Antonio Valle
Antonio
Valle
Gema Cabrera
Gema
Cabrera
Domingo Cantero
Domingo
Cantero
Jorge Bolivar
Jorge
Bolivar
Additional file 8: of Identification of enhanced hydrogen and ethanol Escherichia coli producer strains in a glycerol-based medium by screening in single-knock out mutant collections
Springer Nature
2015
Escherichia coli
Ethanol
Glycerol
High throughput
Hydrogen
2015-06-28 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_8_of_Identification_of_enhanced_hydrogen_and_ethanol_Escherichia_coli_producer_strains_in_a_glycerol-based_medium_by_screening_in_single-knock_out_mutant_collections/4410293
Table S2. The primers from 1 to 6 were used to obtain multiple mutants; ldhAgnd:kan (M2), ldhAgndfrdBC::kan (M4) and ldhAgndfrdBCtdcE::kan (M5). Capital letters indicate the homologous sequences of the target genes and in lower case letters indicate the priming sequences of the pKD13 vector. In the names of the primers, H2P1 indicates the forward primers and H1P4 the reverse primers. Primers from 7 to 15 were used for PCR verification of the deletion of the targeted genes.