10.6084/m9.figshare.c.3626126_D12.v1
Gaëlle Lelandais
Gaëlle
Lelandais
Ivo Scheiber
Ivo
Scheiber
Javier Paz-Yepes
Javier
Paz-Yepes
Jean-Claude Lozano
Jean-Claude
Lozano
Hugo Botebol
Hugo
Botebol
Jana Pilátová
Jana
Pilátová
Vojtěch Žárský
Vojtěch
Žárský
Thibaut Léger
Thibaut
Léger
Pierre-Louis Blaiseau
Pierre-Louis
Blaiseau
Chris Bowler
Chris
Bowler
François-Yves Bouget
François-Yves
Bouget
Jean-Michel Camadro
Jean-Michel
Camadro
Robert Sutak
Robert
Sutak
Emmanuel Lesuisse
Emmanuel
Lesuisse
Additional file 13: Figure S11. of
Ostreococcus tauri is a new model green alga for studying iron metabolism in eukaryotic phytoplankton
Springer Nature
2016
Iron
Ostreococcus
Marine phytoplankton
RNA-seq analysis
2016-05-03 05:00:00
Presentation
https://springernature.figshare.com/articles/presentation/Additional_file_13_Figure_S11_of___Ostreococcus_tauri_is_a_new_model_green_alga_for_studying_iron_metabolism_in_eukaryotic_phytoplankton/4409360
Mass spectrometry analysis of the proteins present in the iron-containing band of the native gel shown in Fig. 5. A: LC-MSMS-based Ostreococcus Protein identification from in-gel trypsin digestions of 55Fe-labelled proteins (from the iron band shown in Fig. 5). Right and left panels show the results of two independent experiments. Identifications were sorted by descending Mascot scores. Data presented include the Mascot score, the protein sequence coverage (as %), the number of proteins in the identified protein groups, the number of unique peptides, and of peptide search matches, together with the description of the protein in terms of number of aminoacid residues, molecular mass and calculated isoelectric point. B: MSMS-based OtFea sequence coverage. Peptides identified at a 1 % FDR are highlighted in green. C: Representative annotated fragmentation spectrum of an OtFea peptide (Charge: +2, Monoisotopic m/z: 795.88043 Da (−0.27 mmu/-0.34 ppm), MH+: 1590.75359 Da, RT: 27.91 min) and D: corresponding sequence attribution (AQALVADGGVCETGSR, C11-Carbamidomethyl (57.02146 Da)). The peptide was identified with Mascot (v1.30) with an ion Score of 83 and an e-value of 8.2E-008. Fragment match tolerance used for search was 0.02 Da. Fragments used for search were: a; a-H2O; a-NH3; b; b-H2O; b-NH3; y; y-H2O; y-NH3. (PPTX 659 kb)